FpCzf14 is a putative C2H2 transcription factor regulating conidiation in Fusarium pseudograminearum

Phytopathology Research

Bingjian Sun¹  Yuming Ma¹  Yankun Liu¹  Yan Shi¹  Jingya Zhao¹  Huiqing Xia¹  Mengya Peng¹  Xiaoping Xing¹  Honglian Li²  Linlin Chen² 

[1] College of Plant Protection, Henan Agricultural University, 450000, Zhengzhou, China;College of Plant Protection, Henan Agricultural University, 450000, Zhengzhou, China;National Key Laboratory of Wheat and Maize Crop Science, 450000, Zhengzhou, China;

关键词: Fusarium pseudograminearum;    Transcription factors;    FpCZF14;    Conidiation;    Pathogenicity;   

DOI  :  10.1186/s42483-020-00074-7

来源: Springer

【 摘 要 】

C2H2 zinc finger transcription factors such as FlbC and Msn2, have broad regulatory roles in fungal growth and conidiation. In the present study, we cloned and characterized a C2H2 zinc finger transcription factor gene, FpCzf14, in the wheat pathogen Fusarium pseudograminearum. FpCzf14 was localized to the nuclei. The expression of FpCzf14 was significantly upregulated in conidia, suggesting that FpCzf14 might contribute to conidiation. Further analysis of the fpczf14-deleted mutant (Δfpczf14) demonstrated that it exhibited defect in conidiation, and this defect was restored in the complemented strain Δfpczf14-C expressing FpCzf14, demonstrating that FpCzf14 was essential for conidiation. Moreover, FpCzf14 was required for mycelial growth and pathogenicity of F. pseudograminearum. Microscopic observation results showed that Δfpczf14 produced only very few penetration pegs and invasive hyphae inside host tissues compared with WT and Δfpczf14-C. Additionally, results of reverse transcription quantitative PCR (RT-qPCR) showed that FpCzf14 regulated expression of several conidiation-related genes in F. pseudograminearum. In conclusion, FpCzf14, as a core regulatory gene in conidiation, provides new insights into the mechanism of conidiation in F. pseudograminearum.

【 授权许可】

CC BY