MicroRNA-296-5p inhibits cell metastasis and invasion in nasopharyngeal carcinoma by reversing transforming growth factor-β-induced epithelial–mesenchymal transition

Cellular & Molecular Biology Letters

Haiqing Luo¹  Jing Ren²  Qiuqin Dai²  Keyuan Zhou²  Xudong Tang²  Chen Chen²  Wenjia Hu²  Xiangyong Li²  Meihui Chen³ 

[1] Center of Oncology of The Affiliated Hospital of Guangdong Medical University, 524023, Zhanjiang, China;Institute of Biochemistry and Molecular Biology of Guangdong Medical University, No. 2 Wenming Dong Road, Xiashan District, 524023, Zhanjiang, Guangdong, China;Institute of Biochemistry and Molecular Biology of Guangdong Medical University, No. 2 Wenming Dong Road, Xiashan District, 524023, Zhanjiang, Guangdong, China;Department of Clinical Laboratory of Zhanjiang Central Hospital, 524023, Zhanjiang, China;

关键词: miR-296-5p;    Metastasis;    Invasion;    EMT;    Nasopharyngeal carcinoma;   

DOI  :  10.1186/s11658-020-00240-x

来源: Springer

【 摘 要 】

AimTo explore the effect of miR-296-5p on the metastasis of nasopharyngeal carcinoma (NPC) cells and investigate the underlying mechanism.MethodsThe expressions of miR-296-5p in NPC tissues and cells were determined using GSE32920 database analysis and real-time PCR and miRNA microarray assays. An miR-296-5p mimic and inhibitor were transfected into NPC cells. Then, immunofluorescence imaging, scratch wound-healing, transwell migration and invasion assays were used to observe the effects of miR-296-5p on cell metastasis and invasion. Real-time PCR and western blotting were carried out to detect the expressions of genes and proteins related to epithelial–mesenchymal transition (EMT). A dual luciferase reporter assay was used to identify whether TGF-β is the target gene of miR-296-5p. Finally, TGF-β expression plasmids were transfected into NPC cells to verify the role of TGF-β in the miR-296-5p-mediated inhibition of nasopharyngeal carcinoma cell metastasis.ResultsOur results show that miR-296-5p inhibits the migratory and invasive capacities of NPC cells by targeting TGF-β, which suppresses EMT. Importantly, the miR-296-5p level was significantly lower in human NPC tissues than in adjacent normal tissues. It also negatively correlated with TGF-β and was significantly associated with the lymph node metastasis of patients with NPC.ConclusionsOur findings show that miR-296-5p represses the EMT-related metastasis of NPC by targeting TGF-β. This provides new insight into the role of miR-296-5p in regulating NPC metastasis and invasiveness.

【 授权许可】

CC BY