Functional analysis and evaluation of respiratory cilia in healthy Chinese children

Respiratory Research

So-Lun Lee¹  Yu-Lung Lau²  Chun-Wai Davy Lee²  Christopher O’Callaghan³ 

[1] Department of Paediatrics and Adolescent Medicine, Queen Mary Hospital, HKSAR, China;Department of Paediatrics and Adolescent Medicine, Duchess of Kent Children’s Hospital, HKSAR, China;Department of Paediatrics and Adolescent Medicine, The University of Hong Kong, HKSAR, China;Respiratory, Critical Care and Anaesthesia, UCL Great Ormond Street Institute of Child Health and GOSH NIHR BRC, London, UK;

关键词: Beat frequency;    Beat pattern;    Chinese children;    Nasal cilia;    Reference data;    Ultrastructure;   

DOI  :  10.1186/s12931-020-01506-w

来源: Springer

【 摘 要 】

BackgroundTo aid in the diagnosis of Primary Ciliary Dyskinesia (PCD) and to evaluate the respiratory epithelium in respiratory disease, normal age-related reference ranges are needed for ciliary beat frequency (CBF), beat pattern and ultrastructure. Our aim was to establish reference ranges for healthy Chinese children.MethodsCiliated epithelial samples were obtained from 135 healthy Chinese children aged below 18 years by brushing the inferior nasal turbinate. CBF and beat pattern were analysed from high speed video recordings. Epithelial integrity and ciliary ultrastructure were assessed using transmission electronic microscopy.ResultsThe mean CBF from 135 children studied was 10.1 Hz (95% CI 9.8 to 10.4). Approximately 20% (ranged 18.0–24.2%) of ciliated epithelial edges were found to have areas of dyskinetically beating cilia. Normal beat pattern was observed in ciliated epithelium from all subjects. We did not find any effect of exposure to second hand smoke on CBF in our subjects. Microtubular defects were found in 9.3% of all of the cilia counted in these children, while other ciliary ultrastructural defects were found in less than 3%.ConclusionsWe established the reference range for CBF, beat pattern and ultrastructure in healthy Chinese children. Using similar methodology, we found a lower overall mean CBF than previously obtained European values. This study highlights the need to establish normative data for ciliary function in different populations.

【 授权许可】

CC BY