期刊论文详细信息
BMC Genomics
Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature
Margaret Y. Gruber1  Justin Renaud2  Muhammad Arshad3  Abdelali Hannoufa4  Aaron J. Simkovich4  Alpa Puri4  Frédéric Marsolais4 
[1] Agriculture and Agri-Food Canada, 107 Science Place, S7N 0X2, Saskatoon, Saskatchewan, Canada;Agriculture and Agri-Food Canada, 1391 Sandford Street, N5V 4T3, London, Ontario, Canada;Agriculture and Agri-Food Canada, 1391 Sandford Street, N5V 4T3, London, Ontario, Canada;Centre for Genomics and Systems Biology, New York University, Abu Dhabi, United Arab Emirates;Agriculture and Agri-Food Canada, 1391 Sandford Street, N5V 4T3, London, Ontario, Canada;Department of Biology, University of Western Ontario, 1151 Richmond Street, N6A 5B7, London, Ontario, Canada;
关键词: Alfalfa;    Heat stress;    miR156;    Proteomic;    LC-MS/MS;   
DOI  :  10.1186/s12864-020-07161-1
来源: Springer
PDF
【 摘 要 】

BackgroundAbiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level.ResultsIn this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 °C) for 24 h. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free quantification proteomics. Across all samples, a total of 1878 protein groups were detected. Under heat stress, 45 protein groups in the empty vector plants were significantly altered (P < 0.05; |log2FC| > 2). Conversely, 105 protein groups were significantly altered when miR156OE alfalfa was subjected to heat stress, of which 91 were unique to miR156OE plants. The identified protein groups unique to miR156OE plants were related to diverse functions including metabolism, photosynthesis, stress-response and plant defenses. Furthermore, we identified transcription factors in miR156OE plants, which belonged to squamosa promoter binding-like protein, MYB, ethylene responsive factors, AP2 domain, ABA response element binding factor and bZIP families of transcription factors.ConclusionsThese results suggest a positive role for miR156 in heat stress response in alfalfa. They reveal a miR156-regulated network of mechanisms at the protein level to modulate heat responses in alfalfa.

【 授权许可】

CC BY   

【 预 览 】
附件列表
Files Size Format View
RO202104285526700ZK.pdf 2150KB PDF download
  文献评价指标  
  下载次数:8次 浏览次数:1次