Characterization of poplar growth-regulating factors and analysis of their function in leaf size control

BMC Plant Biology

Yanqiu Zhao¹  Jinnan Wang¹  Pengbo Sun¹  Fang Tang²  Xueqin Song²  Meng-Zhu Lu³  Houjun Zhou⁴ 

[1] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, 100091, Beijing, China;State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, 100091, Beijing, China;Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China;State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, 100091, Beijing, China;Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 210037, Nanjing, China;Zhejiang Agriculture & Forestry University, 311300, Hangzhou, China;State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, 100091, Beijing, China;Ludong University, 264025, Yantai, China;

关键词: Growth-regulating factor;    Phylogenetic relationship;    miR396;    Leaf development;    Populus;   

DOI  :  10.1186/s12870-020-02699-4

来源: Springer

【 摘 要 】

BackgroundGrowth-regulating factors (GRFs) are plant-specific transcription factors that control organ size. Nineteen GRF genes were identified in the Populus trichocarpa genome and one was reported to control leaf size mainly by regulating cell expansion. In this study, we further characterize the roles of the other poplar GRFs in leaf size control in a similar manner.ResultsThe 19 poplar GRF genes were clustered into six groups according to their phylogenetic relationship with Arabidopsis GRFs. Bioinformatic analysis, degradome, and transient transcription assays showed that 18 poplar GRFs were regulated by miR396, with GRF12b the only exception. The functions of PagGRF6b (Pag, Populus alba × P. glandulosa), PagGRF7a, PagGRF12a, and PagGRF12b, representing three different groups, were investigated. The results show that PagGRF6b may have no function on leaf size control, while PagGRF7a functions as a negative regulator of leaf size by regulating cell expansion. By contrast, PagGRF12a and PagGRF12b may function as positive regulators of leaf size control by regulating both cell proliferation and expansion, primarily cell proliferation.ConclusionsThe diversity of poplar GRFs in leaf size control may facilitate the specific, coordinated regulation of poplar leaf development through fine adjustment of cell proliferation and expansion.

【 授权许可】

CC BY