Serological determinants of COVID-19

Biology Direct

Andrea Duggento¹  Cartesio D’Agostini²  Ivano Amelio³  Maria Luisa Santoro⁴  Annalisa Noce⁵  Nicola Di Daniele⁵  Manfredi Tesauro⁵  Giulia Marrone⁶ 

[1] Department of Biomedicine and Prevention, University of Rome Tor Vergata, via Montpellier 1, 00133, Rome, Italy;Department of Experimental Medicine, University of Rome Tor Vergata, via Montpellier 1, 00133, Rome, Italy;Laboratory of Clinical Microbiology, Policlinico Tor Vergata, viale Oxford 81, 00133, Rome, Italy;Department of Experimental Medicine, University of Rome Tor Vergata, via Montpellier 1, 00133, Rome, Italy;School of Life Sciences, University of Nottingham, Nottingham, UK;Laboratory Pathologist Director of Artemisia Lab – Alessandria, Via Piave, 76 00187, Rome, Italy;UOC of Internal Medicine-Center of Hypertension and Nephrology Unit, Department of Systems Medicine, University of Rome Tor Vergata, via Montpellier 1, 00133, Rome, Italy;UOC of Internal Medicine-Center of Hypertension and Nephrology Unit, Department of Systems Medicine, University of Rome Tor Vergata, via Montpellier 1, 00133, Rome, Italy;PhD School of Applied Medical, Surgical Sciences, University of Rome Tor Vergata, via Montpellier 1, 00133, Rome, Italy;

关键词: SARS-CoV-2;    COVID-19;    Point of care;    Lateral flow immunoassay;    Automated chemiluminescent immunoassay;    Serological tests;    Laboratory detection;   

DOI  :  10.1186/s13062-020-00276-1

来源: Springer

【 摘 要 】

BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection spreaded rapidly worldwide, as far as it has become a global pandemic. Therefore, the introduction of serological tests for determination of IgM and IgG antibodies has become the main diagnostic tool, useful for tracking the spread of the virus and for consequently allowing its containment. In our study we compared point of care test (POCT) lateral flow immunoassay (FIA) vs automated chemiluminescent immunoassay (CLIA), in order to assess their specificity and sensibility for COVID-19 antibodies detection.ResultsWe find that different specificities and sensitivities for IgM and IgG tests. Notably IgM POCT FIA method vs CLIA method (gold standard) has a low sensitivity (0.526), while IgG POCT FIA method vs CLIA method (gold standard) test has a much higher sensitivity (0.937); further, with respect of IgG, FIA and CLIA could arguably provide equivalent information.ConclusionsFIA method could be helpful in assessing in short time, the possible contagiousness of subjects that for work reasons cannot guarantee “social distancing”.

【 授权许可】

CC BY