Evaluation of TaqMan Array card (TAC) for the detection of 28 respiratory pathogens

BMC Infectious Diseases

Hongbo Jing¹  Shuiping Chen²  Ying Chen³  Yongqiang Jiang⁴  Decong Kong⁴  Peng Liu⁴  Yvling Zheng⁴  Xin Zheng⁴  Hua Jiang⁴  Keke Liu⁵ 

[1] Department of Laboratory Medicine, Shunyi District Center for Disease Control and Prevention, Beijing, China;Department of Laboratory Medicine, the Fifth Medical Centre, Chinese PLA General Hospital, Beijing, China;School of Food and Chemical Engineering, Beijing Technology and Business University, 100048, Beijing, China;State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China;State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China;Shandong Academy of Clinical Medicine, Shandong Provincial Hospital, 250021, Jinan, China;

关键词: Respiratory pathogen;    TaqMan Array card;    Real-time PCR;    Nucleic acid detection;   

DOI  :  10.1186/s12879-020-05562-x

来源: Springer

【 摘 要 】

BackgroundRespiratory infections are a serious threat to human health. So, rapid detection of all respiratory pathogens can facilitate prompt treatment and prevent the deterioration of respiratory disease. Previously published primers and probes of the TaqMan array card (TAC) for respiratory pathogens are not sensitive to Chinese clinical specimens. This study aimed to develop and improve the TAC assay to detect 28 respiratory viral and bacterial pathogens in a Chinese population.MethodsTo improve the sensitivity, we redesigned the primers and probes, and labeled the probes with minor groove binders. The amplification efficiency, sensitivity, and specificity of the primers and probes were determined using target-gene containing standard plasmids. The detection performance of the TAC was evaluated on 754 clinical specimens and the results were compared with those from conventional methods.ResultsThe performance of the TAC assay was evaluated using 754 clinical throat swab samples and the results were compared with those from gold-standard methods. The sensitivity and specificity were 95.4 and 96.6%, respectively. The lowest detection limit of the TAC was 10 to 100 copies/μL.ConclusionsTAC is an efficient, accurate, and high-throughput approach to detecting multiple respiratory pathogens simultaneously and is a promising tool for the identification of pathogen outbreaks.

【 授权许可】

CC BY