Draft genome assembly of Passalora sequoiae a needle blight pathogen on Leyland cypress

BMC Research Notes

Jorge Ibarra Caballero¹  Jane E. Stewart¹  Alan S. Windham²  Renee S. Arias³  Valerie A. Orner³  Warren E. Copes⁴  Ebrahiem Babiker⁴ 

[1] Dept. of Agricultural Biology, Colorado State University, 80523, Fort Collins, CO, USA;Soil, Plant and Pest Center, University of Tennessee, 37211, Nashville, TN, USA;USDA ARS National Peanut Research Laboratory, 39842, Dawson, GA, USA;USDA ARS Thad Cochran Southern Horticultural Laboratory, 39470, Poplarville, MS, USA;

关键词: Cupressocyparis leylandii;    Genome annotation;    Illumina;    Leyland cypress;    Needle blight;    PacBio;   

DOI  :  10.1186/s13104-020-05328-3

来源: Springer

【 摘 要 】

ObjectivePassalora sequoiae (family Mycosphaerellaceae) causes a twig blight on Leyland cypress that requires numerous fungicide applications annually to minimize economic losses for ornamental plant nursery and Christmas tree producers. The objective was to generate a high-quality draft assembly of the genome of P. sequoiae as a resource for primer development to investigate genotype diversity.Data descriptionWe report here the genome sequence of P. sequoiae 9LC2 that was isolated from Leyland cypress ‘Leighton Green’ in 2017 in southern Mississippi, USA. The draft genome was obtained using Pacific Biosciences (PacBio) SMRT and Illumina HiSeq 2500 sequencing. Illumina reads were mapped to PacBio assembled contigs to determine base call consistency. Based on a total of 44 contigs with 722 kilobase (kb) average length (range 9.4 kb to 3.4 Mb), the whole genome size was estimated at 31,768,716 bp. Mapping of Illumina reads to PacBio contigs resulted in a 1000 × coverage and were used to confirm accuracy of the consensus sequences.

【 授权许可】

CC BY