Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis

BMC Plant Biology

Lei Wang¹  Xiangyong Li¹  Danxia Ke²  Yuefeng Guan³  Dezhen Qiu⁴  Hongli Yang⁴  Zhihui Shan⁴  Dong Cao⁴  Shuilian Chen⁴  Qingnan Hao⁴  Haifeng Chen⁴  Rong Li⁴  Wei Guo⁴  Chanjuan Zhang⁴  Xinan Zhou⁴  Yi Huang⁴  Limiao Chen⁴  Songli Yuan⁴  Xiaojuan Zhang⁴  Zhonglu Yang⁴ 

[1] College of Life Sciences & Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China;College of Resources and Environment, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, China;College of Life Sciences & Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China;FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Fujian Agriculture and Forestry University, 350002, Fuzhou, China;Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, China;

关键词: Genome-wide survey;    Nodule development and senescence;    Papain-like cysteine protease;    Root nodule symbiosis;    Soybean;   

DOI  :  10.1186/s12870-020-02725-5

来源: Springer

【 摘 要 】

BackgroundPlant papain-like cysteine proteases (PLCPs) are a large class of proteolytic enzymes and play important roles in root nodule symbiosis (RNS), while the whole-genome studies of PLCP family genes in legume are quite limited, and the roles of Glycine max PLCPs (GmPLCPs) in nodulation, nodule development and senescence are not fully understood.ResultsIn the present study, we identified 97 GmPLCPs and performed a genome-wide survey to explore the expansion of soybean PLCP family genes and their relationships to RNS. Nineteen paralogous pairs of genomic segments, consisting of 77 GmPLCPs, formed by whole-genome duplication (WGD) events were identified, showing a high degree of complexity in duplication. Phylogenetic analysis among different species showed that the lineage differentiation of GmPLCPs occurred after family expansion, and large tandem repeat segment were specifically in soybean. The expression patterns of GmPLCPs in symbiosis-related tissues and nodules identified RNS-related GmPLCPs and provided insights into their putative symbiotic functions in soybean. The symbiotic function analyses showed that a RNS-related GmPLCP gene (Glyma.04G190700) really participate in nodulation and nodule development.ConclusionsOur findings improved our understanding of the functional diversity of legume PLCP family genes, and provided insights into the putative roles of the legume PLCPs in nodulation, nodule development and senescence.

【 授权许可】

CC BY