Efficacy of low-dose local clindamycin in different times for microbial decontamination of autogenous particulate bone graft

International Journal of Implant Dentistry

Faramarz Masjedian¹  Gholamreza Irajian¹  Reza Tabrizi²  Hassan Mohajerani²  Fatemeh Latifi² 

[1] Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran;Department of Oral and Maxillofacial Surgery, Shahid Beheshti University of Medical Sciences, Velenjak, Tehran, Iran;

关键词: Decontamination;    Bone;    Graft;    Clindamycin;    Microorganism;   

DOI  :  10.1186/s40729-020-00263-1

来源: Springer

【 摘 要 】

BackgroundClindamycin in low concentration (20 μg/mL) is safe for vitality and osteogenic potential of bone cells. The aim of this study was to evaluate the efficacy of local clindamycin (20 μg/mL) in two different exposure times, for microbial decontamination of particulate bone graft, collected during implant site preparation.This non-randomized parallel-group study was conducted on samples from 17 patients. The particulate bone collected during implant site preparation was divided into three portions by weight: in group S1, the particulate bone was immersed in thioglycolate broth without any antibiotic treatment; in group S2, the collected particulate bone was irrigated with 100 mL clindamycin solution (20 μg/mL); and in group S3, the collected particulate bone was soaked in one ml clindamycin solution (20 μg/mL) for 3 min. Samples in the three groups were cultured in aerobic and anaerobic media and species and CFU count of isolated bacteria were determined.ResultsAnalysis of the data demonstrated a significant difference among the three groups in the mean count of total microorganisms (P = 0.001). The difference in the mean count of anaerobic and aerobic microorganisms in the three groups was statistically significant as well (P = 0.001). Pseudomonas aeruginosa was the only microorganism that was not affected with the mentioned antibiotic.ConclusionsLocal use of low-dose clindamycin (20 μg/mL)—irrigation or 3 min immersing—is effective for the decontamination of particulate bone grafts.

【 授权许可】

CC BY