Bovine adapted transmissible mink encephalopathy is similar to L-BSE after passage through sheep with the VRQ/VRQ genotype but not VRQ/ARQ

BMC Veterinary Research

Martin Groschup¹  Anne Balkema-Buschmann¹  Eric Nicholson²  Justin Greenlee²  Robyn Kokemuller²  Sarah Jo Moore³  Eric Cassmann³ 

[1] Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, —Isle of Riems, Greifswald, Germany;Virus and Prion Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, P.O. Box 70, 1920 Dayton Avenue, 50010, Ames, IA, USA;Virus and Prion Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, P.O. Box 70, 1920 Dayton Avenue, 50010, Ames, IA, USA;Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-SC0014664, Oak Ridge, USA;

关键词: TME;    Transmissible mink encephalopathy;    L-BSE;    Prion diseases;    Prion;    PRNP;    PrPSc;    Sheep;    TSE;    Transmissible spongiform encephalopathies;    C-BSE;   

DOI  :  10.1186/s12917-020-02611-0

来源: Springer

【 摘 要 】

BackgroundTransmissible mink encephalopathy (TME) is a fatal neurologic disease of farmed mink. Evidence indicates that TME and L-BSE are similar and may be linked in some outbreaks of TME. We previously transmitted bovine adapted TME (bTME) to sheep. The present study compared ovine passaged bTME (o-bTME) to C-BSE and L-BSE in transgenic mice expressing wild type bovine prion protein (TgBovXV). To directly compare the transmission efficiency of all prion strains in this study, we considered the attack rates and mean incubation periods. Additional methods for strain comparison were utilized including lesion profiles, fibril stability, and western blotting.ResultsSheep donor genotype elicited variable disease phenotypes in bovinized mice. Inoculum derived from a sheep with the VRQ/VRQ genotype (o-bTMEVV) resulted in an attack rate, incubation period, western blot profile, and neuropathology most similar to bTME and L-BSE. Conversely, donor material from a sheep with the VRQ/ARQ genotype (o-bTMEAV) elicited a phenotype distinct from o-bTMEVV, bTME and L-BSE. The TSE with the highest transmission efficiency in bovinized mice was L-BSE. The tendency to efficiently transmit to TgBovXV mice decreased in the order bTME, C-BSE, o-bTMEVV, and o-bTMEAV. The transmission efficiency of L-BSE was approximately 1.3 times higher than o-bTMEVV and 3.2 times higher than o-bTMEAV.ConclusionsOur findings provide insight on how sheep host genotype modulates strain genesis and influences interspecies transmission characteristics. Given that the transmission efficiencies of L-BSE and bTME are higher than C-BSE, coupled with previous reports of L-BSE transmission to mice expressing the human prion protein, continued monitoring for atypical BSE is advisable in order to prevent occurrences of interspecies transmission that may affect humans or other species.

【 授权许可】

CC BY