Isolation and characterization of Uropathogenic Escherichia coli (UPEC) from red panda (Ailurus fulgens)

BMC Veterinary Research

Xin Chen¹  Chanjuan Yue¹  Xiaoyan Su¹  Yunli Li¹  Xia Yan¹  Kuixing Yang¹  Dongsheng Zhang¹  Rong Hou¹  Guifu Zhuo¹  Songrui Liu¹  Tong Cai²  Xi Peng²  Jiangfeng Liu² 

[1] Chengdu Research Base of Giant Panda Breeding, 610081, Chengdu, Sichuan, China;Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, 610081, Chengdu, Sichuan, China;Sichuan Academy of Giant Panda, 610081, Chengdu, Sichuan, China;Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), 637009, Nanchong, Sichuan, China;College of Life Science, China West Normal University, 637009, Nanchong, Sichuan, China;

关键词: Red panda;    Escherichia coli;    Pathological diagnosis;   

DOI  :  10.1186/s12917-020-02624-9

来源: Springer

【 摘 要 】

BackgroundDisease prevention and control is a significant part in the ex-situ conservation of the endangered red panda (Ailurus fulgens), being bacterial infection is one of the most important health threats to the captive population. To date, studies about the infection caused by Escherichia coli in the red panda are scarce. This study was conducted to determine the cause of death of a captive red panda through clinical symptoms, complete blood count, biochemical analysis, pathological diagnosis and bacterial whole genome sequencing.Case presentationThe following report describes a case of a 1.5 year old captive red panda (Ailurus fulgens) that was found lethargic and anorectic. She was moved to the quarantine area for daily treatment with 50 mg of Cefpodoxime Proxetil. During the three-day treatment, she did not eat or defecate, and then died. Clinical hematology revealed the values of neutrophils, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and blood urea nitrogen (BUN) were significantly higher. Histological analysis demonstrated major pathological damage in the kidneys, liver and lungs, characterized by hyperemia, parenchymal cell degeneration and necrosis and inflammatory cell infiltration which were predominantly neutrophilic. A bacterial strain confirmed as Escherichia coli was isolated post mortem. Whole genome sequencing of the E. coli showed the complete genome size was 4.99 Mbp. PapA, PapC, OmpA, OmpU and other virulence factors which specific to Uropathogenic Escherichia coli (UPEC) were found in the isolate. Among the virulence factors, P pili, type I pili and related factors of the iron uptake system were associated with nephrotoxicity.ConclusionThe red panda died of bacterial infection caused by an uropathogenic strain of Escherichia coli. The pathogenic mechanisms of the strain are closely related to the expression of specific virulence genes.

【 授权许可】

CC BY