Investigation of epididymal proteins and general sperm membrane characteristics of Formosan pangolin (Manis pentadactyla pentadactyla)

BMC Zoology

Shih-Chien Chin¹  Ting-Yu Chen¹  Tse-En Wang²  Yu-Syuan Wei³  Pei-Shiue Tsai⁴  Jane-Fang Yu⁵  Yu-Chia Chang⁶ 

[1] Animal medical center, Taipei Zoo, 30 Xinguang Road, Section 2, Wenshan, 11656, Taipei, Taiwan;Graduate Institute of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, 10617, Taipei, Taiwan;Graduate Institute of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, 10617, Taipei, Taiwan;Department of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, 10617, Taipei, Taiwan;Graduate Institute of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, 10617, Taipei, Taiwan;Department of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, 10617, Taipei, Taiwan;Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, 10617, Taipei, Taiwan;Reproductive Science Lab, Conservation and Research Center, Taipei Zoo, 30 Xinguang Road, Section 2, Wenshan, 11656, Taipei, Taiwan;Animal medical center, Taipei Zoo, 30 Xinguang Road, Section 2, Wenshan, 11656, Taipei, Taiwan;Reproductive Science Lab, Conservation and Research Center, Taipei Zoo, 30 Xinguang Road, Section 2, Wenshan, 11656, Taipei, Taiwan;Graduate Institute of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, 10617, Taipei, Taiwan;

关键词: Pangolin;    Epididymis;    Sperm;    Reproduction;    V-ATPase;   

DOI  :  10.1186/s40850-020-00064-4

来源: Springer

【 摘 要 】

BackgroundFormosan Pangolin (Manis pentadactyla pentadactyla) is one of the three subspecies of Chinese pangolins, it is also an isolated sub-species naturally habitat in Taiwan. Despite earlier report on successful breeding of Sunda (Manis javanica) pangolin, breeding of Formosan pangolins in zoo captive populations is still challenging due to unknown reproductive characterizations of this species in both male and female populations.ResultsWe characterized for the first time, reproductive tract of male Formosan pangolin. We showed pangolin epididymis was a collagen-enriched organ with apparent segmented sub-regions similar to other mammals. However, unlike most mammals exhibited two V-ATPase subunits, Formosan pangolin exhibited only V-ATPase subunit 2. This specific V-ATPase subunit extended its cellular localization throughout the cytoplasm of epididymal clear cells, suggesting pH regulation of luminal microenvironment might be different from other mammals. Electron micrographs showed rod-shaped pangolin sperm cells with multi-lamellar membrane structure at the sperm head. Similar to well-defined capacitation and acrosome reaction membrane changes in other mammals, we reported three distinct patterns (homogenous, punctuated and faded) of pangolin sperm head membrane changes. The concurrent increase in phosphotyrosine protein expression detected at the sperm mid-piece/tail and the emergence of punctuated membrane aggregates likely representing three sperm activation stages, namely inactivated, capacitated and acrosome reacted status of pangolin sperm.ConclusionBy revealing unique epididymal V-ATPase distribution and sperm membrane dynamics in Formosan pangolin, we would understand better the fundamental aspects of reproduction parameters of Formosan pangolin.

【 授权许可】

CC BY