TUG1 enhances high glucose-impaired endothelial progenitor cell function via miR-29c-3p/PDGF-BB/Wnt signaling

Stem Cell Research & Therapy

Shilong Han¹  Maoquan Li¹  Haijun Zhang¹  Yang Li¹  Xue Li¹  Weishuai Lian¹  Xiaoping Zhang¹  Kangkang Zhi² 

[1] Department of Interventional & Vascular Surgery, Tenth People’s Hospital of Tongji University, 200072, Shanghai, China;Institute of Interventional & Vascular Surgery, Tongji University, 200072, Shanghai, China;Department of Vascular and Endovascular Surgery, Changzheng Hospital, 200003, Shanghai, China;

关键词: lncRNA taurine upregulated gene 1;    Platelet-derived growth factor-BB;    Endothelial progenitor cells;    Diabetes;    Angiogenesis;   

DOI  :  10.1186/s13287-020-01958-3

来源: Springer

【 摘 要 】

BackgroundDiabetes is associated with the dysfunction of endothelial progenitor cells (EPCs), characterized as impaired angiogenesis, a phenomenon thought to be involved in the development of diabetic foot. lncRNA plays an essential role in microvascular dysfunction and signaling pathways in patients with diabetes. lncRNA taurine upregulated gene 1 (TUG1) participates in angiogenesis in various cells. However, the mechanisms of TUG1 activity in EPCs have not been elucidated.MethodsWe isolated and then characterized EPCs from the peripheral blood of mice using immunofluorescence and flow cytometry. Western blot detected the wnt/β-catenin pathway in high glucose-treated EPCs. Bioinformatics analysis predicted a putative binding site for TUG1 on miR-29c-3p. The interactions among TUG1, platelet-derived growth factor-BB (PDGF-BB), and miR-29c-3p were analyzed by luciferase assays. In vivo, diabetic mouse ischemic limb was treated with normal saline or TUG1 overexpression lentiviruses.ResultsWe found that EPC migration, invasion, and tube formation declined after treatment with high glucose, but improved with TUG1 overexpression. Mechanically, wnt/β-catenin pathway and autophagy were involved in the function of TUG1 overexpression in high glucose-treated EPCs. Moreover, TUG1 regulates the PDGF-BB/wnt pathway and function of high glucose-treated EPCs via miR-29c-3p. In vivo, injection of TUG1 lentivirus in a diabetic mouse ischemic limb model stimulated angiogenesis.ConclusionsOur findings suggest that TUG1 restores high glucose-treated EPC function by regulating miR-29c-3p/PDGF-BB/Wnt signaling.

【 授权许可】

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