Serial blockface SEM suggests that stem cells may participate in adult notochord growth in an invertebrate chordate, the Bahamas lancelet

EvoDevo

Nicholas D. Holland¹  Ildiko M. L. Somorjai² 

[1] Marine Biology Research Division, Scripps Institution of Oceanography, University of California At San Diego, 92093, La Jolla, CA, USA;School of Biology, University of Saint Andrews, KY16 9ST, St. Andrews, Scotland;

关键词: Cephalochordata;    Lancelet;    Amphioxus;    Serial blockface scanning electron microscopy (SBSEM);    Notochord;    Stem cell;    Progenitor cell;    Intervertebral disc;    Nucleus pulposus;   

DOI  :  10.1186/s13227-020-00167-6

来源: Springer

【 摘 要 】

BackgroundThe cellular basis of adult growth in cephalochordates (lancelets or amphioxus) has received little attention. Lancelets and their constituent organs grow slowly but continuously during adult life. Here, we consider whether this slow organ growth involves tissue-specific stem cells. Specifically, we focus on the cell populations in the notochord of an adult lancelet and use serial blockface scanning electron microscopy (SBSEM) to reconstruct the three-dimensional fine structure of all the cells in a tissue volume considerably larger than normally imaged with this technique.ResultsIn the notochordal region studied, we identified 10 cells with stem cell-like morphology at the posterior tip of the organ, 160 progenitor (Müller) cells arranged along its surface, and 385 highly differentiated lamellar cells constituting its core. Each cell type could clearly be distinguished on the basis of cytoplasmic density and overall cell shape. Moreover, because of the large sample size, transitions between cell types were obvious.ConclusionsFor the notochord of adult lancelets, a reasonable interpretation of our data indicates growth of the organ is based on stem cells that self-renew and also give rise to progenitor cells that, in turn, differentiate into lamellar cells. Our discussion compares the cellular basis of adult notochord growth among chordates in general. In the vertebrates, several studies implied that proliferating cells (chordoblasts) in the cortex of the organ might be stem cells. However, we think it is more likely that such cells actually constitute a progenitor population downstream from and maintained by inconspicuous stem cells. We venture to suggest that careful searches should find stem cells in the adult notochords of many vertebrates, although possibly not in the notochordal vestiges (nucleus pulposus regions) of mammals, where the presence of endogenous proliferating cells remains controversial.

【 授权许可】

CC BY