Electrochemical detection of white spot syndrome virus with a silicone rubber disposable electrode composed of graphene quantum dots and gold nanoparticle-embedded polyaniline nanowires

Journal of Nanobiotechnology

Sungjo Park¹  Jun Satoh²  Jirayu Boonyakida³  Kenshin Takemura³  Enoch Y. Park⁴  Ankan Dutta Chowdhury⁵ 

[1] Division of Cardiovascular Diseases, Mayo Clinic College of Medicine and Science, Mayo Clinic, 200 First Street SW, 55905, Rochester, MN, USA;Division of Pathology, Department of Aquaculture Research, Fisheries Technology Institute of Japan Fisheries Research and Education Agency, National Research and Development Agency, Tamaki Field Station, 224-1 Hiruta, Tamaki, 519-0423, Watarai, Mie, Japan;Laboratory of Biotechnology, Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, 422-8529, Shizuoka, Japan;Laboratory of Biotechnology, Department of Bioscience, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, 422-8529, Shizuoka, Japan;Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, 422-8529, Shizuoka, Japan;Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, 422-8529, Shizuoka, Japan;

关键词: White spot syndrome virus;    Electrochemical virus detection;    Disposable electrode;    Polyaniline;    Gold nanoparticle;    Graphene quantum dots;   

DOI  :  10.1186/s12951-020-00712-4

来源: Springer

【 摘 要 】

BackgroundWith the enormous increment of globalization and global warming, it is expected that the number of newly evolved infectious diseases will continue to increase. To prevent damage due to these infections, the development of a diagnostic method for detecting a virus with high sensitivity in a short time is highly desired. In this study, we have developed a disposable electrode with high-sensitivity and accuracy to evaluate its performances for several target viruses.ResultsConductive silicon rubber (CSR) was used to fabricate a disposable sensing matrix composed of nitrogen and sulfur-co-doped graphene quantum dots (N,S-GQDs) and a gold-polyaniline nanocomposite (AuNP-PAni). A specific anti-white spot syndrome virus (WSSV) antibody was conjugated to the surface of this nanocomposite, which was successfully applied for the detection of WSSV over a wide linear range of concentration from 1.45 × 102 to 1.45 × 105 DNA copies/ml, with a detection limit as low as 48.4 DNA copies/ml.ConclusionThe engineered sensor electrode can retain the detection activity up to 5 weeks, to confirm its long-term stability, required for disposable sensing applications. This is the first demonstration of the detection of WSSV by a nanofabricated sensing electrode with high sensitivity, selectivity, and stability, providing as a potential diagnostic tool to monitor WSSV in the aquaculture industry.

【 授权许可】

CC BY