Lysophosphatidic acid-RAGE axis promotes lung and mammary oncogenesis via protein kinase B and regulating tumor microenvironment

Cell Communication and Signaling

Satyendra Kumar Singh¹  Vivek Rai¹  Rashmi Ray¹  Nitish Jangde²  Sunita Sinha² 

[1] Laboratory of Vascular Immunology, Institute of Life Sciences, (An Autonomous Institute of Department of Biotechnology (DBT) New Delhi), 751023, Bhubaneswar, India;Laboratory of Vascular Immunology, Institute of Life Sciences, (An Autonomous Institute of Department of Biotechnology (DBT) New Delhi), 751023, Bhubaneswar, India;Manipal Academy of Higher Education, Karnataka-576104, Manipal, India;

关键词: Cancer;    Tumorigenesis;    Metastasis;    Migration;    Invasion;   

DOI  :  10.1186/s12964-020-00666-y

来源: Springer

【 摘 要 】

BackgroundReceptor for advanced glycation end products (RAGE) is a multi-ligand transmembrane receptor of the immunoglobulin superfamily. Lysophosphatidic acid (LPA) is a ligand for RAGE and is involved in physiological and pathophysiological conditions including cancer. However, RAGE-LPA axis is unexplored in lung and mammary cancer.MethodsRAGE was silenced in A549, MDA MB-231 and MCF7 using RAGE shRNA. For in vitro tumorigenesis, we performed wound healing, colony formation, cell proliferation and invasion assays. Evaluation of expression of oncogenes, EMT markers and downstream signaling molecules was done by using western blot and immunohistochemistry. For subcellular expression of RAGE, immunofluorescence was done. In vivo tumorigenesis was assessed by intraperitoneal injection of cancer cells in nude mice.ResultsHere we show RAGE mediated profound increase in proliferation, migration and invasion of lung and mammary cancer cells via LPA in Protein kinase B (PKB) dependent manner. LPA mediated EMT transition is regulated by RAGE. In vivo xenograft results show significance of RAGE in LPA mediated lung and mammary tumor progression, angiogenesis and immune cell infiltration to tumor microenvironment.ConclusionOur results establish the significance and involvement of RAGE in LPA mediated lung and mammary tumor progression and EMT transition via RAGE. RAGE-LPA axis may be a therapeutic target in lung and mammary cancer treatment strategies.EdVB1f1yrz4Do4i3nWt_5KVideo Abstract

【 授权许可】

CC BY