Genome-wide analysis of differentially expressed mRNAs, lncRNAs, and circRNAs in chicken bursae of Fabricius during infection with very virulent infectious bursal disease virus

BMC Genomics

Meng Wang¹  Zengsu Liu¹  Xuewei Huang¹  Junyan Zhang¹  Xiaolong Fan¹  Han Zhou²  Lijie Tang²  Li Wang²  Yanping Jiang²  Xinyuan Qiao²  Yijing Li²  Wen Cui²  Yigang Xu² 

[1]College of Veterinary Medicine, Northeast Agricultural University, Changjiang Road No. 600, Xiang Fang District, Harbin, People’s Republic of China

[2]College of Veterinary Medicine, Northeast Agricultural University, Changjiang Road No. 600, Xiang Fang District, Harbin, People’s Republic of China

[3]Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, People’s Republic of China

关键词: Infectious bursal disease virus;    Bursa of Fabricius;    mRNAs;    lncRNAs;    circRNAs;   

DOI  :  10.1186/s12864-020-07129-1

来源: Springer

【 摘 要 】

BackgroundInfectious bursal disease virus (IBDV) causes acute, highly contagious, immunosuppressive, and lethal infectious disease in young chickens and mainly infects the bursa of Fabricius (BF). To investigate interactions between IBDV and its host, RNA sequencing was applied to analyze the responses of the differentially expressed transcriptional profiles of BF infected by very virulent IBDV (vvIBDV).ResultsIn total, 317 upregulated and 94 downregulated mRNAs were found to be significantly differentially expressed in infected chickens, compared to controls. Long non-coding RNA (lncRNA) and circular RNA (circRNA) alterations were identified in IBDV-infected chickens, and significantly different expression was observed in 272 lncRNAs and 143 circRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed to assess the functions of significantly dysregulated genes, which showed that the JAK-STAT signaling pathway, the NOD-like receptor signaling pathway, and apoptosis may be activated by IBDV infection. We predicted interactions between differentially expressed genes and produced lncRNA-mRNA and circRNA-miRNA-mRNA regulator network.ConclusionsThe present study identified the expression profiles of mRNAs, lncRNAs, and circRNAs during vvIBDV infection and provides new insights into the pathogenesis of IBDV and antiviral immunity of the host.

【 授权许可】

CC BY