miRNA-23b as a biomarker of culture-positive neonatal sepsis

Molecular Medicine

José Santiago Ibáñez-Cabellos¹  José Luis García-Giménez¹  Federico V. Pallardó¹  Nafissa Chabni²  Ahlam Fatmi³  Hafsa Azzaoui³  Mourad Aribi³  Yamina Elhabiri⁴  Sid Ahmed Rebiahi⁴  Hanane Zerrouki⁴  Souheila Benmassour⁵  Mohammed Chems-Eddine Smahi⁵ 

[1] Center for Biomedical Network Research on Rare Diseases (CIBERER), Institute of Health Carlos III, Valencia, Spain;INCLIVA Health Research Institute, Mixed Unit for rare diseases INCLIVA-CIPF, Valencia, Spain;Department of Physiology, Faculty of Medicine and Dentistry, University of Valencia, Avenida Blasco Ibañez 15, 46010, Valencia, Spain;Faculty of Medicine, Tlemcen Medical Centre University, 13000, Tlemcen, Algeria;Laboratory of Applied Molecular Biology and Immunology, W0414100, Tlemcen, Algeria;Laboratory of Microbiology Applied in Food, Biomedical and Environment, Tlemcen, Algeria;Neonatal Department of Specialized Maternal and Child Hospital of Tlemcen, 13000, Tlemcen, Algeria;

关键词: Early-onset sepsis;    Haemoculture;    Late-onset sepsis;    miR-23b;    Newborns;   

DOI  :  10.1186/s10020-020-00217-8

来源: Springer

【 摘 要 】

BackgroundNeonatal sepsis remains an important cause of morbidity and mortality. The ability to quickly and accurately diagnose neonatal sepsis based on clinical assessments and laboratory blood tests remains difficult, where haemoculture is the gold standard for detecting bacterial sepsis in blood culture. It is also very difficult to study because neonatal samples are lacking.MethodsForty-eight newborns suspected of sepsis admitted to the Neonatology Department of the Mother-Child Specialized Hospital of Tlemcen. From each newborn, a minimum of 1–2 ml of blood was drawn by standard sterile procedures for blood culture. The miRNA-23b level in haemoculture was evaluated by RT-qPCR.ResultsmiR-23b levels increased in premature and full-term newborns in early onset sepsis (p < 0.001 and p < 0.005 respectively), but lowered in late onset sepsis in full-term neonates (p < 0.05) compared to the respective negative controls. miR-23b levels also increased in late sepsis in the negative versus early sepsis negative controls (p < 0.05). miR-23b levels significantly lowered in the newborns who died from both sepsis types (p < 0.0001 and p < 0.05 respectively). In early sepsis, miR-23b and death strongly and negatively correlated (correlation coefficient = − 0.96, p = 0.0019). In late sepsis, miRNA-23b and number of survivors (correlation coefficient = 0.70, p = 0.506) positively correlated.ConclusionsLowering miR-23b levels is an important factor that favours sepsis development, which would confirm their vital protective role, and strongly suggest that they act as a good marker in molecular diagnosis and patient monitoring.

【 授权许可】

CC BY