Untargeted metabolomics reveals the effect of lovastatin on steroid-induced necrosis of the femoral head in rabbits

Journal of Orthopaedic Surgery and Research

Zixing Shao¹  Xiangnan Ren²  Zixuan Wang³  Kaiyun Chen⁴  Wu Fan⁴  Xuefeng Yu⁴ 

[1]Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, 100084, Beijing, China

[2]Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, 100084, Beijing, China

[3]Beijing Institute of Nutritional Resources, 100069, Beijing, China

[4]Nanchang University, 330006, Nanchang, China

[5]The Fourth Affiliated Hospital, Nanchang University, 330003, Nanchang, China

关键词: Lovastatin;    Metabolomics;    Femoral head necrosis;    Steroid-induced;   

DOI  :  10.1186/s13018-020-02026-5

来源: Springer

【 摘 要 】

PurposeLovastatin is an important medicine and it shows a significant effect against glucocorticoid-induced necrosis of the femoral head. This study aimed to investigate the effect of lovastatin on preventing necrosis of the femoral head of by serum metabolomics strategy.MethodsAdult healthy adult Japanese white rabbits were divided into three groups: control group, model group, and drug group. The pathologic changes of femoral head were assessed with magnetic resonance imaging and microscope. Metabolomics based on ultra-high performance liquid chromatography tandem mass spectrometry analysis was used to analyze the collected serum sample. Data were analyzed using principal component analysis, partial least squares-discriminate analysis, and orthogonal partial least squares-discriminant analysis. All potential metabolites were identified by comparing with human metabolome database, Metlin database, lipid maps, and chemspider database.ResultsEleven potential biomarkers were noted and identified as potential biomarkers. The change of biomarkers suggested that lovastatin on preventing necrosis of the femoral head may affect glycerophospholipid metabolism, linoleic acid metabolism, sphingolipid metabolism, alpha-linolenic acid metabolism, pyrimidine metabolism, and arachidonic acid metabolism.ConclusionThe study suggested that lovastatin could prevent the glucocorticoid-induced necrosis of the femoral head of rabbits. The possible reasons were closely associated with adjusting the lipid metabolism, inhibiting adipogenesis, and delaying the osteocyte apoptosis.

【 授权许可】

CC BY