Elevated MTA1 induced the migration and invasion of renal cell carcinoma through the NF-κB pathway

BMC Urology

Yuan Huang¹  Shixing Shen²  Cai Lv³  Qingqing Lei³  Zhenxiang Liu³  Wenxia Si⁴ 

[1] Department of Neurology, Haikou Municipal Hospital, 570208, Haikou, Hainan, China;Department of Urology, Danzhou People’s Hospital, 571799, Danzhou, Hainan, China;Department of Urology, Haikou Municipal Hospital, 570208, Haikou, Hainan, China;Hubei Key Laboratory for Kidney Disease Pathogenesis and Intervention, Huangshi Central Hospital of Edong Healthcare Group, Hubei Polytechnic University School of Medicine, Huangshi, China;

关键词: MTA1;    RCC;    Cell migration and invasion;    NF-κB;    MMP2/MMP9;    E-cadherin;   

DOI  :  10.1186/s12894-020-00731-1

来源: Springer

【 摘 要 】

BackgroundThe metastasis-associated gene 1 (MTA1) has been extensively reported as a crucial oncogene, and its abnormal expression has been associated with the progression of numerous cancers. However, the role of MTA1 in renal cell carcinoma (RCC) progression and metastasis remains unclear. Herein, we investigated the expression of MTA1 and its role in RCC.Methods109 matched clear cell RCCs (ccRCCs) and corresponding normal tissue samples were analyzed via immunohistochemistry to test the expression of MTA1. Human A498 cell lines were transfected with pcDNA3.1-Flag (control) or Flag-MTA1 to overexpress MTA1 or with specific interfering RNA (si-MTA1) or specific interfering negative control to knockdown MTA1 expression. Transfected cells were used in wound healing and transwell invasion assay. Quantitative real time polymerase chain reaction was used to assess the effect of MTA1 on MMP2/MMP9 and E-cadherin gene expression. Western blot was used to qualify the phosphorylation of p65.ResultsHerein, we found a significantly increased expression of MTA1 in 109 ccRCCs, compared to the corresponding normal tissue. In addition, the overexpression of MTA1 in A498 cells facilitated cell migration and invasion, while the down-regulation of MTA1 expression using specific interfering RNA sequences could decrease cell migration and invasion. Furthermore, we showed that MTA1 is up-regulated in ccRCCs, which contributes to the migration and invasion of human kidney cancer cells by mediating the expression of MMP2 and MMP9 through the NF-κB signaling pathway. Similarly, we found that MTA1 could regulate E-cadherin expression in RCCs.ConclusionsMTA1 is overexpressed in RCC and is involved in the progression of RCC through NF-κB.

【 授权许可】

CC BY