Analysis of the role of the human papillomavirus 16/18 E7 protein assay in screening for cervical intraepithelial neoplasia: a case control study

BMC Cancer

Shuhui Song¹  Moudu Liu¹  Huiping Lou²  Pengfei Liu²  Xiaoping Xiao³  Ying Zhang³  Lan Zhu³  Qingbo Fan³  Yan Li³  Honghui Shi³  Linghua Kong³  Ruoli Guan³  Haiyuan Liu³  Xin Yu³  Caijuan Li³  Tao Xu⁴ 

[1] AMID Biotech (Tianjin) Co., Ltd., No. 4, Haitai Development 2nd Road, Binhai Gaoxin District, 300450, Tianjin, China;Department of Health Sciences, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Shuaifuyuan No. 1, Dongcheng District, 100730, Beijing, China;Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Shuaifuyuan No. 1, Dongcheng District, 100730, Beijing, China;Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, 100005, Beijing, China;

关键词: Cervical intraepithelial neoplasia (CIN);    E7 oncoprotein;    High-risk human papillomavirus (Hr-HPV);   

DOI  :  10.1186/s12885-020-07483-z

来源: Springer

【 摘 要 】

BackgroundCervical cancer is the second-most common gynecological cancer, early screening plays a key role in the diagnosis and treatment of cervical intraepithelial neoplasia (CIN). Sustained E7 protein expression is the pathological basis for CIN and cervical cancer.MethodsWe collected the cervical cell samples of women who visited the gynecological clinic of Peking Union Medical College Hospital between September 2018 and September 2019 and submitted them to the high-risk human papillomavirus (Hr-HPV) test. We performed a magnetic particle–based chemiluminescence enzyme immunoassay to analyze the HPV16/18 E7 protein level in CIN of different severities and compared the results with those of cervical pathology (gold standard) and the HPV test.ResultsThe positive rate of HPV16/18 E7 protein increased with the severity of CIN: 26.6% in normal tissue, 58.3% in CIN1, and 70.6% in CIN2 or higher (CIN2+). For CIN2+, the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the E7 protein were 70.6, 67.9, 52.2, and 82.3%, respectively. These values of the HPV test were 86.8, 44.5, 43.7, and 87.1%, respectively. With the combination of the E7 protein assay and HPV test, the specificity for diagnosing CIN2+ was 78.1%, which was significantly higher than that of the HPV test alone.ConclusionsHPV16/18 E7 protein level is correlated with the severity of CIN and has a high concordance rate with the pathological result. For cervical cancer screening, the combination of HPV16/18 E7 protein assay and HPV test improves the CIN diagnostic specificity, detection rate, and detection accuracy.

【 授权许可】

CC BY