An integrative phylogenomic approach to elucidate the evolutionary history and divergence times of Neuropterida (Insecta: Holometabola)

BMC Evolutionary Biology

Christoph Mayer¹  Alexander Donath¹  Lars Podsiadlowski¹  Doria Lieberz¹  Alexandros Vasilikopoulos¹  Bernhard Misof¹  Karen Meusemann²  Ralph S. Peters³  Xin Zhou⁴  Shanlin Liu⁴  Xingyue Liu⁴  Oliver Niehuis⁵  Alexander Böhm⁶  Daniela Bartel⁶  Ulrike Aspöck⁷  Tomáš Flouri⁸  Paschalia Kapli⁸  Rolf G. Beutel⁹  Horst Aspöck^1,10  Carola Greve^1,11  Torsten Wappler^1,12  James E. Jepson^1,13  Jes Rust^1,14 

[1] Centre for Molecular Biodiversity Research, Zoological Research Museum Alexander Koenig, 53113, Bonn, Germany;Centre for Molecular Biodiversity Research, Zoological Research Museum Alexander Koenig, 53113, Bonn, Germany;Department of Evolutionary Biology and Ecology, Institute of Biology I (Zoology), Albert-Ludwigs-Universität Freiburg, 79104, Freiburg, Germany;Australian National Insect Collection, National Research Collections Australia, Commonwealth Scientific and Industrial Research Organisation (CSIRO), ACT 2601, Canberra, Australia;Centre for Taxonomy and Evolutionary Research, Arthropoda Department, Zoological Research Museum Alexander Koenig, 53113, Bonn, Germany;Department of Entomology, China Agricultural University, 100193, Beijing, People’s Republic of China;Department of Evolutionary Biology and Ecology, Institute of Biology I (Zoology), Albert-Ludwigs-Universität Freiburg, 79104, Freiburg, Germany;Department of Evolutionary Biology, University of Vienna, 1090, Vienna, Austria;Department of Evolutionary Biology, University of Vienna, 1090, Vienna, Austria;Zoological Department II, Natural History Museum of Vienna, 1010, Vienna, Austria;Department of Genetics, Evolution and Environment, University College London, WC1E 6BT, London, UK;Institut für Zoologie und Evolutionsforschung, Friedrich-Schiller-Universität Jena, 07743, Jena, Germany;Institute of Specific Prophylaxis and Tropical Medicine, Medical Parasitology, Medical University of Vienna (MUW), 1090, Vienna, Austria;LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), 60325, Frankfurt, Germany;Natural History Department, Hessisches Landesmuseum Darmstadt, 64283, Darmstadt, Germany;School of Biological, Earth and Environmental Sciences, University College Cork, Distillery Fields, North Mall, T23 N73K, Cork, Ireland;Steinmann-Institut für Geologie, Mineralogie und Paläontologie, Rheinische Friedrich-Wilhelms-Universität Bonn, 53115, Bonn, Germany;

关键词: Megaloptera;    Neuroptera;    Raphidioptera;    Endopterygota;    Transcriptomics;    RNA-seq;    Multi-species coalescent;    Supermatrices;    Four-cluster likelihood mapping;   

DOI  :  10.1186/s12862-020-01631-6

来源: Springer

【 摘 要 】

BackgroundThe latest advancements in DNA sequencing technologies have facilitated the resolution of the phylogeny of insects, yet parts of the tree of Holometabola remain unresolved. The phylogeny of Neuropterida has been extensively studied, but no strong consensus exists concerning the phylogenetic relationships within the order Neuroptera. Here, we assembled a novel transcriptomic dataset to address previously unresolved issues in the phylogeny of Neuropterida and to infer divergence times within the group. We tested the robustness of our phylogenetic estimates by comparing summary coalescent and concatenation-based phylogenetic approaches and by employing different quartet-based measures of phylogenomic incongruence, combined with data permutations.ResultsOur results suggest that the order Raphidioptera is sister to Neuroptera + Megaloptera. Coniopterygidae is inferred as sister to all remaining neuropteran families suggesting that larval cryptonephry could be a ground plan feature of Neuroptera. A clade that includes Nevrorthidae, Osmylidae, and Sisyridae (i.e. Osmyloidea) is inferred as sister to all other Neuroptera except Coniopterygidae, and Dilaridae is placed as sister to all remaining neuropteran families. Ithonidae is inferred as the sister group of monophyletic Myrmeleontiformia. The phylogenetic affinities of Chrysopidae and Hemerobiidae were dependent on the data type analyzed, and quartet-based analyses showed only weak support for the placement of Hemerobiidae as sister to Ithonidae + Myrmeleontiformia. Our molecular dating analyses suggest that most families of Neuropterida started to diversify in the Jurassic and our ancestral character state reconstructions suggest a primarily terrestrial environment of the larvae of Neuropterida and Neuroptera.ConclusionOur extensive phylogenomic analyses consolidate several key aspects in the backbone phylogeny of Neuropterida, such as the basal placement of Coniopterygidae within Neuroptera and the monophyly of Osmyloidea. Furthermore, they provide new insights into the timing of diversification of Neuropterida. Despite the vast amount of analyzed molecular data, we found that certain nodes in the tree of Neuroptera are not robustly resolved. Therefore, we emphasize the importance of integrating the results of morphological analyses with those of sequence-based phylogenomics. We also suggest that comparative analyses of genomic meta-characters should be incorporated into future phylogenomic studies of Neuropterida.

【 授权许可】

CC BY