7-T MRI of explanted liver and ex-vivo pancreatic specimens: prospective study protocol of radiological-pathological correlation feasibility (the EXLIPSE project)

European Radiology Experimental

Giacomo Aringhieri¹  Francesco Campana¹  Laura Crocetti¹  Rosa Cervelli¹  Ugo Boggi²  Paolo De Simone³  Andrea Cacciato Insilla⁴  Daniela Campani⁴  Matteo Cencini⁵  Guido Buonincontri⁵  Michela Tosetti⁵ 

[1] Division of Diagnostic and Interventional Radiology, University of Pisa, Via Paradisa, 2 – Cisanello Hospital, 56100, Pisa, Italy;Division of General and Transplant and Surgery, University of Pisa, Pisa, Italy;Division of Hepatic Surgery and Liver Transplant, University of Pisa, Pisa, Italy;Division of Pathology, University of Pisa, Pisa, Italy;IMAGO7 Foundation - IRCCS Stella Maris, Pisa, Italy;

关键词: Liver cirrhosis;    Liver steatosis;    Liver transplantation;    Magnetic resonance imaging;    Pancreatic neoplasms;   

DOI  :  10.1186/s41747-020-00185-y

来源: Springer

【 摘 要 】

The study focuses on radiological-pathological correlation between imaging of ex vivo samples obtained by a 7-T scanner and histological examination. The specimens will be derived from native explanted cirrhotic livers, liver grafts excluded from donation because of severe steatosis, and primary pancreatic tumours. Magnetic resonance imaging (MRI) examinations will be performed within 24 h from liver or pancreatic lesion surgical removal. The MRI protocol will include morphological sequences, quantitative T1, T2, and fat-, water-fraction maps with Cartesian k-space acquisition, and multiparametric methods based on a transient-state “MRI fingerprinting”. Finally, the specimen will be fixed by formalin. Qualitative imaging analysis will be performed by two independent blinded radiologists to assess image consistency score. Quantitative analysis will be performed by drawing regions of interest on different tissue zones to measure T1 and T2 relaxation times as well as fat- and water-fraction. The same tissue areas will be analysed by the pathologists. This study will provide the possibility to improve our knowledge about qualitative and quantitative abdominal imaging assessment at 7 T, by correlating imaging characteristics and the corresponding histological composition of ex vivo specimens, in order to identify imaging biomarkers. Trial registration: ClinicalTrials.gov: 13646. Registered 9 July 2019—retrospectively registered

【 授权许可】

CC BY