Commentary on human pluripotent stem cell-based blood–brain barrier models

Fluids and Barriers of the CNS

Samira M. Azarin¹  Sean P. Palecek²  Eric V. Shusta³  Ethan S. Lippmann⁴ 

[1] Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, USA;Department of Chemical and Biological Engineering, University of Wisconsin–Madison, Madison, WI, USA;Department of Chemical and Biological Engineering, University of Wisconsin–Madison, Madison, WI, USA;Department of Neurological Surgery, University of Wisconsin–Madison, Madison, WI, USA;Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA;

关键词: Blood–brain barrier;    Human pluripotent stem cell;    In vitro model;   

DOI  :  10.1186/s12987-020-00222-3

来源: Springer

【 摘 要 】

In 2012, we provided the first published evidence that human pluripotent stem cells could be differentiated to cells exhibiting markers and phenotypes characteristic of the blood–brain barrier (BBB). In the ensuing years, the initial protocols have been refined, and the research community has identified both positive and negative attributes of this stem cell-based BBB model system. Here, we give our perspective on the current status of these models and their use in the BBB community, as well as highlight key attributes that would benefit from improvement moving forward.

【 授权许可】

CC BY