Genome Biology | |
Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize | |
Xiu-Li Han1  Min-Hui Lu1  Yun Zhou2  Caixia Gao3  Qiupeng Lin3  Yuan-Yuan Jiang4  Yu Zhang4  Qiang Zhang4  Yi-Ping Chai4  Xue-Chen Wang4  Qi-Jun Chen5  | |
[1] Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, 100193, Beijing, China;Collaborative Innovation Center of Crop Stress Biology, Henan Province; Institute of Plant Stress Biology, School of Life Science, Henan University, 475004, Kaifeng, China;State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China;College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China;State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100193, Beijing, China;State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100193, Beijing, China;Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, 100193, Beijing, China; | |
关键词: Prime editing; PE2; pegRNA; CRISPR/Cas9; Maize; ALS; Herbicide resistance; | |
DOI : 10.1186/s13059-020-02170-5 | |
来源: Springer | |
【 摘 要 】
Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.
【 授权许可】
CC BY
【 预 览 】
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RO202104269235971ZK.pdf | 2033KB | download |