Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Genome Biology

Xiu-Li Han¹  Min-Hui Lu¹  Yun Zhou²  Caixia Gao³  Qiupeng Lin³  Yuan-Yuan Jiang⁴  Yu Zhang⁴  Qiang Zhang⁴  Yi-Ping Chai⁴  Xue-Chen Wang⁴  Qi-Jun Chen⁵ 

[1] Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, 100193, Beijing, China;Collaborative Innovation Center of Crop Stress Biology, Henan Province; Institute of Plant Stress Biology, School of Life Science, Henan University, 475004, Kaifeng, China;State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China;College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China;State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100193, Beijing, China;State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, 100193, Beijing, China;Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, 100193, Beijing, China;

关键词: Prime editing;    PE2;    pegRNA;    CRISPR/Cas9;    Maize;    ALS;    Herbicide resistance;   

DOI  :  10.1186/s13059-020-02170-5

来源: Springer

【 摘 要 】

Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.

【 授权许可】

CC BY