The recycling endosome protein Rab25 coordinates collective cell movements in the zebrafish surface epithelium

eLife

Isis So¹  Ashley EE Bruce¹  Tianhui Chen¹  Patrick Morley Willoughby¹  Yupeng Liu¹  Roman Korytnikov¹  Neil Macpherson¹  Molly Allen¹  Jennifer A Mitchell¹  Rodrigo Fernandez-Gonzalez²  Jessica Yu³ 

[1] Department of Cell and Systems Biology, University of Toronto, Toronto, Canada;Department of Cell and Systems Biology, University of Toronto, Toronto, Canada;Ted Rogers Centre for Heart Research, Translational Biology and Engineering Program, University of Toronto, Toronto, Canada;Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Canada;Ted Rogers Centre for Heart Research, Translational Biology and Engineering Program, University of Toronto, Toronto, Canada;Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Canada;

关键词: epiboly;    Rabs;    epithelial morphogenesis;    cytokinesis;    trafficking;    abscission;    Zebrafish;   

DOI  :  10.7554/eLife.66060

来源: eLife Sciences Publications, Ltd

【 摘 要 】

In emerging epithelial tissues, cells undergo dramatic rearrangements to promote tissue shape changes. Dividing cells remain interconnected via transient cytokinetic bridges. Bridges are cleaved during abscission and currently, the consequences of disrupting abscission in developing epithelia are not well understood. We show that the Rab GTPase Rab25 localizes near cytokinetic midbodies and likely coordinates abscission through endomembrane trafficking in the epithelium of the zebrafish gastrula during epiboly. In maternal-zygotic Rab25a and Rab25b mutant embryos, morphogenic activity tears open persistent apical cytokinetic bridges that failed to undergo timely abscission. Cytokinesis defects result in anisotropic cell morphologies that are associated with a reduction of contractile actomyosin networks. This slows cell rearrangements and alters the viscoelastic responses of the tissue, all of which likely contribute to delayed epiboly. We present a model in which Rab25 trafficking coordinates cytokinetic bridge abscission and cortical actin density, impacting local cell shape changes and tissue-scale forces.

【 授权许可】

CC BY