Generic injuries are sufficient to induce ectopic Wnt organizers in Hydra

eLife

Jack F Cazet¹  Adrienne Cho¹  Celina E Juliano¹ 

[1] Department of Molecular and Cellular Biology, University of California, Davis, Davis, United States;

关键词: Hydra;    regeneration;    Wnt signaling;    wound repair;    patterning;    transcriptional regulation;    Other;   

DOI  :  10.7554/eLife.60562

来源: eLife Sciences Publications, Ltd

【 摘 要 】

During whole-body regeneration, a bisection injury can trigger two different types of regeneration. To understand the transcriptional regulation underlying this adaptive response, we characterized transcript abundance and chromatin accessibility during oral and aboral regeneration in the cnidarian Hydra vulgaris. We found that the initial response to amputation at both wound sites is identical and includes widespread apoptosis and the activation of the oral-specifying Wnt signaling pathway. By 8 hr post amputation, Wnt signaling became restricted to oral regeneration. Wnt pathway genes were also upregulated in puncture wounds, and these wounds induced the formation of ectopic oral structures if pre-existing organizers were simultaneously amputated. Our work suggests that oral patterning is activated as part of a generic injury response in Hydra, and that alternative injury outcomes are dependent on signals from the surrounding tissue. Furthermore, Wnt signaling is likely part of a conserved wound response predating the split of cnidarians and bilaterians.

【 授权许可】

CC BY