Robust, coherent, and synchronized circadian clock-controlled oscillations along Anabaena filaments

eLife

Francesca Di Patti¹  Rinat Arbel-Goren²  Sergio Camargo²  Anna Zhitnitsky²  Joel Stavans²  Duccio Fanelli³  Valentina Buonfiglio³  Antonia Herrero⁴  Enrique Flores⁴  Ana Valladares⁴ 

[1] Consiglio Nazionale delle Ricerche, Istituto dei Sistemi Complessi, Sesto Fiorentino, Italy;Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot, Israel;Dipartimento di Fisica e Astronomia, Università di Firenze, INFN and CSDC, Sesto Fiorentino, Italy;Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC and Universidad de Sevilla, Sevilla, Spain;

关键词: Circadian clock;    Anabaena;    cell-cell communication;    demographic noise;    stochastic oscillations;    clock arrays;    Other;   

DOI  :  10.7554/eLife.64348

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Circadian clocks display remarkable reliability despite significant stochasticity in biomolecular reactions. We study the dynamics of a circadian clock-controlled gene at the individual cell level in Anabaena sp. PCC 7120, a multicellular filamentous cyanobacterium. We found significant synchronization and spatial coherence along filaments, clock coupling due to cell-cell communication, and gating of the cell cycle. Furthermore, we observed low-amplitude circadian oscillatory transcription of kai genes encoding the post-transcriptional core oscillatory circuit and high-amplitude oscillations of rpaA coding for the master regulator transducing the core clock output. Transcriptional oscillations of rpaA suggest an additional level of regulation. A stochastic one-dimensional toy model of coupled clock cores and their phosphorylation states shows that demographic noise can seed stochastic oscillations outside the region where deterministic limit cycles with circadian periods occur. The model reproduces the observed spatio-temporal coherence along filaments and provides a robust description of coupled circadian clocks in a multicellular organism.

【 授权许可】

CC BY