Assessing the impact of the addition of pyriproxyfen on the durability of permethrin-treated bed nets in Burkina Faso: a compound-randomized controlled trial

Malaria Journal

Alphonse Traoré¹  Alfred B. Tiono¹  Kobié H. Toé¹  N’Falé Sagnon¹  Steve W. Lindsay²  Margaret Pinder³  Emile F. S. Tchicaya⁴  Frank Mechan⁵  James Maas⁵  Hanafy Ismail⁵  Joanna Solino⁵  Marion Morris⁵  Hilary Ranson⁵  Julie-Anne A. Tangena⁵  Natalie Lissenden⁵ 

[1] Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso;Department of Biosciences, Durham University, Durham, UK;Department of Biosciences, Durham University, Durham, UK;Medical Research Council Unit, The Gambia at the London School of Hygiene and Tropical Medicine, Banjul, The Gambia;Swiss Centre for Scientific Research in Côte d’Ivoire, Abidjan, Côte d’Ivoire;Vector Biology Department, Liverpool School of Tropical Medicine, Liverpool, UK;

关键词: Anopheles gambiae;    Burkina Faso;    Long-lasting insecticidal nets;    Malaria control;    Net durability;    Olyset;    Olyset Duo;    Permethrin;    Pyriproxyfen;   

DOI  :  10.1186/s12936-019-3018-1

来源: Springer

【 摘 要 】

BackgroundLong-lasting insecticidal nets (LLINs) treated with pyrethroids are the foundation of malaria control in sub-Saharan Africa. Rising pyrethroid resistance in vectors, however, has driven the development of alternative net formulations. Here the durability of polyethylene nets with a novel combination of a pyrethroid, permethrin, and the insect juvenile hormone mimic, pyriproxyfen (PPF), compared to a standard permethrin LLIN, was assessed in rural Burkina Faso.MethodsA compound-randomized controlled trial was completed in two villages. In one village 326 of the PPF-permethrin nets (Olyset Duo) and 327 standard LLINs (Olyset) were distributed to assess bioefficacy. In a second village, 170 PPF-permethrin nets and 376 LLINs were distributed to assess survivorship. Nets were followed at 6-monthly intervals for 3 years. Bioefficacy was assessed by exposing permethrin-susceptible and resistant Anopheles gambiae sensu lato mosquito strains to standard World Health Organization (WHO) cone and tunnel tests with impacts on fertility measured in the resistant strain. Insecticide content was measured using high-performance liquid chromatography. LLIN survivorship was recorded with a questionnaire and assessed by comparing the physical integrity using the proportionate hole index (pHI).ResultsThe PPF-permethrin net met WHO bioefficacy criteria (≥ 80% mortality or ≥ 95% knockdown) for the first 18 months, compared to 6 months for the standard LLIN. Mean mosquito mortality for PPF-permethrin nets, across all time points, was 8.6% (CI 2.6–14.6%) higher than the standard LLIN. Fertility rates were reduced after PPF-permethrin net exposure at 1-month post distribution, but not later. Permethrin content of both types of nets remained within the target range of 20 g/kg ± 25% for 242/248 nets tested. The pyriproxyfen content of PPF-permethrin nets declined by 54%, from 10.4 g/kg (CI 10.2–10.6) to 4.7 g/kg (CI 3.5–6.0, p < 0.001) over 36 months. Net survivorship was poor, with only 13% of PPF-permethrin nets and 12% of LLINs still present in the original household after 36 months. There was no difference in the fabric integrity or survivorship between the two net types.ConclusionThe PPF-permethrin net, Olyset Duo, met or exceeded the performance of the WHO-recommended standard LLIN (Olyset) in the current study but both net types failed the 3-year WHO bioefficacy criteria.

【 授权许可】

CC BY