Transcriptional profiling of mouse peripheral nerves to the single-cell level to build a sciatic nerve ATlas (SNAT)

eLife

Joanne Gerber¹  Daniel Gerber¹  Jorge A Pereira¹  Ueli Suter¹  Ge Tan²  Emilio Yángüez²  Slavica Dimitrieva² 

[1] Department of Biology, Institute of Molecular Health Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich, Switzerland;Functional Genomics Center Zurich, ETH Zurich/University of Zurich, Zurich, Switzerland;

关键词: Schwann cells;    myelination;    nerves;    peripheral nervous system;    Mouse;   

DOI  :  10.7554/eLife.58591

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Peripheral nerves are organ-like structures containing diverse cell types to optimize function. This interactive assembly includes mostly axon-associated Schwann cells, but also endothelial cells of supporting blood vessels, immune system-associated cells, barrier-forming cells of the perineurium surrounding and protecting nerve fascicles, and connective tissue-resident cells within the intra-fascicular endoneurium and inter-fascicular epineurium. We have established transcriptional profiles of mouse sciatic nerve-inhabitant cells to foster the fundamental understanding of peripheral nerves. To achieve this goal, we have combined bulk RNA sequencing of developing sciatic nerves up to the adult with focused bulk and single-cell RNA sequencing of Schwann cells throughout postnatal development, extended by single-cell transcriptome analysis of the full sciatic nerve both perinatally and in the adult. The results were merged in the transcriptome resource Sciatic Nerve ATlas (SNAT: https://www.snat.ethz.ch). We anticipate that insights gained from our multi-layered analysis will serve as valuable interactive reference point to guide future studies.

【 授权许可】

CC BY