Effect of milk fat-based infant formulae on stool fatty acid soaps and calcium excretion in healthy term infants: two double-blind randomised cross-over trials

BMC Nutrition

Elpis Vlachopapadopoulou¹  Eva Karaglani²  Tarek-Michail Kampani²  Zafeiris Manikas²  Iliana Christaki²  Yannis Manios²  Anastasia Papazoglou²  Eleni Maragoudaki²  Inge Thijs-Verhoeven³  Rolf Bos³  Marlotte M. Vonk³  Panam Parikh³ 

[1] Department of Endocrinology-Growth and Development, Children’s Hospital P. & A. Kyriakou, Athens, Greece;Department of Nutrition and Dietetics, School of Health Science and Education, Harokopio University, Athens, Greece;FrieslandCampina, Stationsplein 1, 3818 LE, Amersfoort, the Netherlands;

关键词: Milk fat;    SN-2-palmitate;    Palmitate soap;    Calcium excretion;    Stool consistency;    Amsterdam infant stool scale;   

DOI  :  10.1186/s40795-020-00365-4

来源: Springer

【 摘 要 】

BackgroundPalmitic acid (PA) is predominantly esterified at the SN-2 position of triacylglycerols in human milk. PA at the SN-2 position is more efficiently absorbed and results in reduced formation of PA soaps, as well as reduced fatty acid (FA) and calcium malabsorption. Bovine milk fat (MF), a natural source of SN-2-palmitate, was used in the fat blend of infant formulae (IF) in the current study to investigate its effect on stool fatty acid soaps, calcium excretion and stool characteristics.MethodsTwo double-blind, randomised cross-over trials (CS1, CS2) were conducted in parallel with healthy term, formula-fed infants aged 9–14 weeks. After a two-week run-in period, infants in CS1 (n = 17) were randomly allocated to receive either a 50% MF-based formula (50MF) or a 100% vegetable fat (VF) formula; in CS2 (n = 18), infants received either a 20% MF-based formula (20MF) or the VF formula, in a 2 × 2-week cross-over design. At the end of each two-week intervention period, stool samples were collected for FA, FA soaps and calcium excretion analysis and stool consistency was assessed according to the Amsterdam Infant Stool Scale (AISS).ResultsMF-based groups showed no significant difference in PA in stools compared to VF group, although reduced stool PA soaps (CS1: 111.28 ± 18.33 vs. 220.25 ± 29.35 mg/g dry weight, p < 0.0001; CS2: 216.24 ± 25.16 vs. 233.94 ± 35.12 mg/g dry weight, p = 0.0023), total FA soaps and calcium excretion (CS1: 46.40 ± 5.27 vs. 49.88 ± 4.77 mg/g dry weight, p = 0.0041; CS2: 46.20 ± 4.26 vs. 50.47 ± 6.71 mg/g dry weight, p = 0.0067) were observed. Furthermore, the 50MF group showed a favourable lower mean stool consistency score compared to the VF group (1.64 ± 0.49 vs. 2.03 ± 0.19, p = 0.0008).ConclusionsWhile the use of bovine MF in IF did not affect PA concentrations in stool, lower excretion of palmitate soaps, total FA soaps and calcium was seen in healthy term infants. 50MF formula also showed improved stool consistency. The use of MF in IF could be an interesting approach to improve gut comfort and stool characteristics in infants, warranting further research.Trial registrationNetherlands Trial Registry Identifier: NTR6702. Date registered: December 01, 2017.

【 授权许可】

CC BY