Dynamic changes of the fecal bacterial community in dairy cows during early lactation

AMB Express

Shoukun Ji¹  Jie Huang²  Feiran Wang³  Shengli Li³  Gibson Maswayi Alugongo³  Shuai Huang³ 

[1] College of Animal Science and Technology, Hebei Agricultural University, 071001, Baoding, China;College of Animal Science and Technology, Yangzhou University, 225009, Yangzhou, China;The State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, China;

关键词: Fecal microbiota;    Fresh dairy cows;    Dynamic;    16S rRNA sequencing;   

DOI  :  10.1186/s13568-020-01106-3

来源: Springer

【 摘 要 】

The dynamics of the community structure and composition of the dairy cow fecal bacterial communities during early lactation is unclear, therefore this study was conducted to characterize the fecal bacterial communities in dairy cows during early lactation using 16S rRNA gene sequencing. Feces were sampled from 20 healthy fresh Holstein dairy cows on day 1 (Fresh1d group) and day 14 (Fresh14d group) after calving. After calving, cows were fed the same fresh diet. The dominant phyla Firmicutes and Proteobacteria were decreased (P ≤ 0.01) with lactating progress and phyla Bacteroidetes were increased (P = 0.008) with lactating progress and dietary transition. At family level, the predominant families were Ruminococcaceae (35.23%), Lachnospiraceae (11.46%), Rikenellaceae (10.44%) and Prevotellaceae (6.89%). A total of 14 genera were different between fecal samples from Fresh1d and Fresh14d, included the predominant genera, such as Ruminococcaceae_UCG-005 (P = 0.008), Rikenellaceae_RC9_gut_group (P = 0.043) and Christensenellaceae_R-7_group (P = 0.008). All fecal bacterial communities shared members of the genera Ruminococcaceae_UCG-005, Bacteroides and Rikenellaceae_RC9_gut_group. These findings help to improve our understanding of the composition and structure of the fecal microbial community in fresh cows and may provide insight into bacterial adaptation time and dietary in lactating cows.

【 授权许可】

CC BY