Effect of diode laser potentiality on proliferation of dental pulp stem cells (in vitro study)

Bulletin of the National Research Centre

Nadia Lashin Soliman¹  Mohamed M. Abo El-Dahab¹  Riham Mohamed Aly²  Mostafa Gheith³ 

[1] Department of Basic Dental Science, Oral & Dental Research Division, National Research Centre, 33 El Buhouth St., Dokki, 12622, Cairo, Egypt;Department of Basic Dental Science, Oral & Dental Research Division, National Research Centre, 33 El Buhouth St., Dokki, 12622, Cairo, Egypt;Stem Cell Laboratory, Center of Excellence for Advanced Sciences, National Research Centre, Cairo, Egypt;National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt;

关键词: Dental pulp stem cells;    Diode laser;    Irradiation;    Proliferation;   

DOI  :  10.1186/s42269-020-00414-9

来源: Springer

【 摘 要 】

BackgroundThe field of laser-based photochemical reactions receives a great promising for additional applications especially for targeting cells, pathogens, or molecules. Limited studies have investigated the impact of light-emitting diode on stem cell behavior. Thus, the aim of the present study was to assess the effect of diode laser irradiations on the proliferation of stem cells isolated from the human dental pulp. Isolation procedures were according to previously developed protocols for dental pulp stem cells (DPSCs). Low-level laser therapy irradiation (LLLT) was applied in two doses (0.5 J/cm2 and 1 J/cm2 for 20 s) into 96-well plates by the diode laser device (970 nm) through the fiber optic (SiroLaser fibers 320) at a distance from the opening of the wells to be accurate for equal distribution of the laser irradiation. To assess the proliferation capacity of the isolated stem cells, MTT assay was performed 24 h, 48 h, and 72 h.ResultsThere was no significant difference among the different groups (gp 1 control, gp II dose 0.5 J/cm2, gp III dose 1 J/cm2) on day 1. While on day 2, the optical density of DPSCs subjected to dose 1 was found to be significantly higher than that of those subjected to dose 2. This was also demonstrated on day 3. It was also demonstrated that the proliferation of DPSCs subjected to dose 1 increased compared to that of DPSCs subjected to dose 2 from day 1 to day 2. There was a significant decrease in the cell number in both groups by 72 h.ConclusionIn conclusion, the use of LLLT as a stimulatory factor for enhancing and proliferation of the stem cells is very promising.

【 授权许可】

CC BY