Bio-organic fertilizers stimulate indigenous soil Pseudomonas populations to enhance plant disease suppression

Microbiome

Stefan Geisen¹  Wu Xiong²  George A. Kowalchuk²  Beibei Wang³  Yunze Ruan³  Rong Li⁴  Shanshan Liu⁴  Zongzhuan Shen⁴  Chengyuan Tao⁴  Qirong Shen⁵ 

[1] Department of Terrestrial Ecology, Netherlands Institute for Ecology, (NIOO-KNAW), 6708, Wageningen, PB, Netherlands;Laboratory of Nematology, Wageningen University, 6700, Wageningen, AA, Netherlands;Ecology and Biodiversity Group, Department of Biology, Institute of Environmental Biology, Utrecht University, 3584, Utrecht, CH, Netherlands;Hainan key Laboratory for Sustainable Utilization of Tropical Bio-resources, College of tropical crops, Hainan University, 570228, Haikou, People’s Republic of China;Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People’s Republic of China;The Key Laboratory of Plant Immunity, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People’s Republic of China;Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People’s Republic of China;The Key Laboratory of Plant Immunity, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People’s Republic of China;College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, Nanjing, People’s Republic of China;

关键词: Bio-organic fertilizer;    Fusarium wilt;    Disease suppression;    Resident microbiota;    Pseudomonas;    Interspecific synergy;   

DOI  :  10.1186/s40168-020-00892-z

来源: Springer

【 摘 要 】

BackgroundPlant diseases caused by fungal pathogen result in a substantial economic impact on the global food and fruit industry. Application of organic fertilizers supplemented with biocontrol microorganisms (i.e. bioorganic fertilizers) has been shown to improve resistance against plant pathogens at least in part due to impacts on the structure and function of the resident soil microbiome. However, it remains unclear whether such improvements are driven by the specific action of microbial inoculants, microbial populations naturally resident to the organic fertilizer or the physical-chemical properties of the compost substrate. The aim of this study was to seek the ecological mechanisms involved in the disease suppressive activity of bio-organic fertilizers.ResultsTo disentangle the mechanism of bio-organic fertilizer action, we conducted an experiment tracking Fusarium wilt disease of banana and changes in soil microbial communities over three growth seasons in response to the following four treatments: bio-organic fertilizer (containing Bacillus amyloliquefaciens W19), organic fertilizer, sterilized organic fertilizer and sterilized organic fertilizer supplemented with B. amyloliquefaciens W19. We found that sterilized bioorganic fertilizer to which Bacillus was re-inoculated provided a similar degree of disease suppression as the non-sterilized bioorganic fertilizer across cropping seasons. We further observed that disease suppression in these treatments is linked to impacts on the resident soil microbial communities, specifically by leading to increases in specific Pseudomonas spp.. Observed correlations between Bacillus amendment and indigenous Pseudomonas spp. that might underlie pathogen suppression were further studied in laboratory and pot experiments. These studies revealed that specific bacterial taxa synergistically increase biofilm formation and likely acted as a plant-beneficial consortium against the pathogen.ConclusionTogether we demonstrate that the action of bioorganic fertilizer is a product of the biocontrol inoculum within the organic amendment and its impact on the resident soil microbiome. This knowledge should help in the design of more efficient biofertilizers designed to promote soil function.CRswXYrqxByVeLx6CQCFERVideo Abstract

【 授权许可】

CC BY