Crystalline silica particles cause rapid NLRP3-dependent mitochondrial depolarization and DNA damage in airway epithelial cells

Particle and Fibre Toxicology

Rongrong Wu¹  Patricia Ramos-Ramírez¹  Huiyuan Zheng¹  Mikael Adner¹  Johan Högberg¹  Ulla Stenius¹ 

[1]Institute of Environmental Medicine, Karolinska Institutet, Box 210, SE-17177, Stockholm, Sweden

关键词: Respirable crystalline silica particles;    NLRP3 inflammasome;    S198 pNLRP3;    Mitochondrial depolarization;    4;    Double strand brakes (DSB);    Non-homologous end joining (NHEJ);    CC10;    GPRC5A;    Autotaxin (ATX);   

DOI  :  10.1186/s12989-020-00370-2

来源: Springer

【 摘 要 】

BackgroundRespirable crystalline silica causes lung carcinomas and many thousand future cancer cases are expected in e.g. Europe. Critical questions are how silica causes genotoxicity in the respiratory epithelium and if new cases can be avoided by lowered permissible exposure levels. In this study we investigate early DNA damaging effects of low doses of silica particles in respiratory epithelial cells in vitro and in vivo in an effort to understand low-dose carcinogenic effects of silica particles.ResultsWe find DNA damage accumulation already after 5–10 min exposure to low doses (5 μg/cm2) of silica particles (Min-U-Sil 5) in vitro. DNA damage was documented as increased levels of γH2AX, pCHK2, by Comet assay, AIM2 induction, and by increased DNA repair (non-homologous end joining) signaling. The DNA damage response (DDR) was not related to increased ROS levels, but to a NLRP3-dependent mitochondrial depolarization. Particles in contact with the plasma membrane elicited a Ser198 phosphorylation of NLRP3, co-localization of NLRP3 to mitochondria and depolarization. FCCP, a mitochondrial uncoupler, as well as overexpressed NLRP3 mimicked the silica-induced depolarization and the DNA damage response. A single inhalation of 25 μg silica particles gave a similar rapid DDR in mouse lung. Biomarkers (CC10 and GPRC5A) indicated an involvement of respiratory epithelial cells.ConclusionsOur findings demonstrate a novel mode of action (MOA) for silica-induced DNA damage and mutagenic double strand breaks in airway epithelial cells. This MOA seems independent of particle uptake and of an involvement of macrophages. Our study might help defining models for estimating exposure levels without DNA damaging effects.

【 授权许可】

CC BY