Reconstruction of lncRNA-miRNA-mRNA network based on competitive endogenous RNA reveals functional lncRNAs in skin cutaneous melanoma

BMC Cancer

Junyou Zhu¹  Ruizhao Cai¹  Jun Wu¹  Shaohai Qi¹  Ning Liu¹  Lijun Zhang¹  Fei Zhou¹  Jingling Zhao¹  Bin Shu¹  Jin Deng² 

[1] Department of Burn, The First Affiliated Hospital, Sun yat-sen University, 510080, Guangzhou, Guangdong, People’s Republic of China;Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, 510095, Guangzhou, Guangdong, People’s Republic of China;

关键词: Human skin cutaneous melanoma;    lncRNA;    Competitive endogenous RNA;    MALAT1;    LINC00943;    LINC00261;    miRNA;   

DOI  :  10.1186/s12885-020-07302-5

来源: Springer

【 摘 要 】

BackgroundHuman skin cutaneous melanoma is the most common and dangerous skin tumour, but its pathogenesis is still unclear. Although some progress has been made in genetic research, no molecular indicators related to the treatment and prognosis of melanoma have been found. In various diseases, dysregulation of lncRNA is common, but its role has not been fully elucidated. In recent years, the birth of the “competitive endogenous RNA” theory has promoted our understanding of lncRNAs.MethodsTo identify the key lncRNAs in melanoma, we reconstructed a global triple network based on the “competitive endogenous RNA” theory. Gene Ontology and KEGG pathway analysis were performed using DAVID (Database for Annotation, Visualization, and Integration Discovery). Our findings were validated through qRT-PCR assays. Moreover, to determine whether the identified hub gene signature is capable of predicting the survival of cutaneous melanoma patients, a multivariate Cox regression model was performed.ResultsAccording to the “competitive endogenous RNA” theory, 898 differentially expressed mRNAs, 53 differentially expressed lncRNAs and 16 differentially expressed miRNAs were selected to reconstruct the competitive endogenous RNA network. MALAT1, LINC00943, and LINC00261 were selected as hub genes and are responsible for the tumorigenesis and prognosis of cutaneous melanoma.ConclusionsMALAT1, LINC00943, and LINC00261 may be closely related to tumorigenesis in cutaneous melanoma. In addition, MALAT1 and LINC00943 may be independent risk factors for the prognosis of patients with this condition and might become predictive molecules for the long-term treatment of melanoma and potential therapeutic targets.

【 授权许可】

CC BY