Genome-wide characterization and expression profiling of MAPK cascade genes in Salvia miltiorrhiza reveals the function of SmMAPK3 and SmMAPK1 in secondary metabolism

BMC Genomics

Meiling Ding¹  Pengda Ma¹  Jie Yang¹  Juane Dong¹  Bin Zhang¹  Yongfeng Xie¹  Tianlin Pei² 

[1] College of Life Sciences, Northwest A&F University, Yangling, China;Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai, China;

关键词: Salvia miltiorrhiza;    Gene family;    MAPK cascades;    Co-expression analysis;    Phenolic acid synthesis;    Tanshinone synthesis;   

DOI  :  10.1186/s12864-020-07023-w

来源: Springer

【 摘 要 】

BackgroundThe contribution of mitogen-activated protein kinase (MAPK) cascades to plant growth and development has been widely studied, but this knowledge has not yet been extended to the medicinal plant Salvia miltiorrhiza, which produces a number of pharmacologically active secondary metabolites.ResultsIn this study, we performed a genome-wide survey and identified six MAPKKK kinases (MAPKKKKs), 83 MAPKK kinases (MAPKKKs), nine MAPK kinases (MAPKKs) and 18 MAPKs in the S. miltiorrhiza genome. Within each class of genes, a small number of subfamilies were recognized. A transcriptional analysis revealed differences in the genes’ behaviour with respect to both their site of transcription and their inducibility by elicitors and phytohormones. Two genes were identified as strong candidates for playing roles in phytohormone signalling. A gene-to-metabolite network was constructed based on correlation analysis, highlighting the likely involvement of two of the cascades in the synthesis of two key groups of pharmacologically active secondary metabolites: phenolic acids and tanshinones.ConclusionThe data provide insight into the functional diversification and conservation of MAPK cascades in S. miltiorrhiza.

【 授权许可】

CC BY