Attenuated lncRNA NKILA Enhances the Secretory Function of Airway Epithelial Cells Stimulated by Mycoplasma pneumoniae via NF-κB

BioMed research international

Jiamin Zhang¹  Yifan Zhu¹  Haiyan Gu¹  Deyu Zhao¹  Yao Zhou¹  Feng Liu¹  Fengxia Zhang²  Qingning Duan³  Yun Guo⁴ 

[1] Department of Respiratory Medicine, Children’s Hospital of Nanjing Medical University, Nanjing 210008, China, njmu.edu.cn;Department of Respiratory Medicine, Children’s Hospital of Nanjing Medical University, Nanjing 210008, China, njmu.edu.cn;Department of Emergency Medicine, Children’s Hospital of Nanjing Medical University, Nanjing 210008, China, njmu.edu.cn;Department of Respiratory Medicine, Children’s Hospital of Nanjing Medical University, Nanjing 210008, China, njmu.edu.cn;Department of Pediatrics, Taizhou People’s Hospital, Taizhou 225700, China, jstzhospital.com;Department of Respiratory Medicine, Children’s Hospital of Nanjing Medical University, Nanjing 210008, China, njmu.edu.cn;Department of Respiratory Medicine, Wuxi Children’s Hospital, Wuxi 214000, China, njmu.edu.cn;

DOI  :  10.1155/2021/6656298

来源: Hindawi Publishing Corporation

【 摘 要 】

The secretory function of airway epithelial cells is important in the pathogenesis of Mycoplasma pneumoniae pneumonia (MPP). To investigate the regulatory function of NKILA (nuclear factor-κB (NF-κB) interacting long noncoding RNA (lncRNA)) in MPP, we first detected NKILA as well as the concentration of interleukin 8 (IL-8) and tumor necrosis factor-α (TNF-α) in bronchoalveolar lavage fluid of children with MPP. Then, NKILA was knocked down in epithelial cells to investigate its effect on their secretory function. The results suggested that NKILA was downregulated in children with MPP, while IL-8 and TNF-α levels increased. Knockdown of NKILA in vitro promoted the inflammatory effects of Mycoplasma pneumoniae (MP) in epithelial A549 and BEAS-2B cells. Knockdown of NKILA promoted inhibitor of κBα (IκBα) phosphorylation and degradation, and NF-κB p65 nuclear translocation. Furthermore, RNA immunoprecipitation showed that NKILA could physically bind to IκBα in MP-treated A549 cells. Collectively, our data demonstrated that attenuation of NKILA enhances the effects of MP-stimulated secretory functions of epithelial cells via regulation of NF-κB signaling.

【 授权许可】

CC BY