In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations

Horticultura Brasileira

Ricardo T. De Faria¹  Fabiana N. Rodrigues¹  Luciana Do V.r. Oliveira¹  Cláudio Müller¹ 

[1] ,UELLondrina PR

关键词: Dendrobium nobile;    Orchidaceae;    carbohydrate;    tissue culture;    Dendrobium nobile;    orchidaceae;    carboidrato;    cultura de tecidos;   

DOI  :  10.1590/S0102-05362004000400023

来源: SciELO

【 摘 要 】

Sucrose is a very important component in in vitro culture media, serving as a source of carbon and energy. In this paper, the rooting and in vitro growth of Dendrobium nobile Lindl (Orchidaceae) were studied using different sucrose concentrations (0 g L-1; 5 g L-1; 10 g L-1; 20 g L-1; 30 g L-1 and 60 g L-1), in a modified MS medium containing half the regular concentration of macronutrients at pH 5.8. Greater increases in plant height (4.21±0.6 cm) and high seedling multiplication (1:4) were observed in the 60 g L-1 sucrose treatment, even without the addition of plant hormones. Sucrose concentration in the culture medium did not influence in vitro plant rooting.

【 授权许可】

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