Evaluation of the antischistosomal activity of sulfated α-D-glucan from the lichen Ramalina celastri free and encapsulated into liposomes

Brazilian Journal of Medical and Biological Research

R.v.s. Araújo²  M.r. Melo-júnior²  E.i.c. Beltrão²  L.a. Mello¹  M. Iacomini¹  A.m.a. Carneiro-leão¹  L.b. Carvalho Jr²  N.s. Santos-magalhães² 

[1] ,Universidade Federal de Pernambuco Laboratório de Imunopatologia Keizo-Asami Recife PE ,Brasil

关键词: Polysaccharide;    α-D-glucan;    Ramalina celastri;    Liposomes;    Schistosoma mansoni;    Lectin histochemistry;   

DOI  :  

来源: SciELO

【 摘 要 】

The antischistosomal activity of the sulfated polysaccharide α-D-glucan (Glu.SO4) extracted from Ramalina celastri was evaluated after encapsulation into liposomes (Glu.SO4-LIPO) in Schistosoma mansoni-infected mice. The effect of treatment with Glu.SO4 and Glu.SO4-LIPO (10 mg/kg) on egg elimination, worm burden and hepatic granuloma formation was assessed using female albino Swiss mice, 35-40 days of age, weighing 25 ± 2 g, infected with 150 cercariae/animal (Biomphalaria glabrata, BH strain). Four groups (N = 10) were studied, two controls (empty liposomes and NaCl) and two treated groups (Glu.SO4-LIPO and Glu.SO4) using a single dose. Parasitological analysis revealed that Glu.SO4-LIPO was as efficient as Glu.SO4 in reducing egg elimination and worm burden. Treatment with free Glu.SO4 and Glu.SO4-LIPO induced a statistically significant reduction in the number of granulomas (62 and 63%, respectively). Lectin histochemistry showed that wheat germ agglutinin intensely stained the egg-granuloma system in all treated groups. On the other hand, peanut agglutinin stained cells in the control groups, but not in the treated groups. The present results suggest a correlation between the decreasing number of hepatic egg-granulomas and the glycosylation profile of the egg-granuloma system in animals treated with free Glu.SO4 or Glu.SO4-LIPO.

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