Different biochemical strategies of two Neotropical fish to cope with the impairment of nitrogen excretion during air exposure

Brazilian Journal of Medical and Biological Research

V.l.p. Polez¹  G. Moraes¹  C. Santos Neto² 

[1] ,Universidade Federal de São Carlos Departamento de Genética e Evolução

关键词: Hoplias malabaricus;    Hoplerythrinus unitaeniatus;    Fish;    Urea cycle enzymes;    Biochemical adaptation;    Air exposure;   

DOI  :  10.1590/S0100-879X2003000200017

来源: SciELO

【 摘 要 】

The exposure of fish to air is normally expected to interfere with the nitrogen excretion process. Hoplias malabaricus and Hoplerythrinus unitaeniatus, two teleost species, display distinct behaviors in response to decreases in natural reservoir water levels, although they may employ similar biochemical strategies. To investigate this point, plasma levels of ammonia, urea, uric acid, and the two urea cycle enzymes, ornithine carbamoyl transferase (OCT) and arginase (ARG), as well as glutamine synthetase (GS) were determined for both species after exposure to air. Plasma ammonia increased gradually during exposure to air, but only H. malabaricus showed increased concentrations of urea. Plasma uric acid remained very low in both fish. Enzymatic activities (mean ± SD, µmol min-1 g protein-1) of H. malabaricus showed significant increases (P<0.05, N = 6) in OCT from 0.84 ± 0.05 to 1.42 ± 0.03, in ARG from 8.07 ± 0.47 to 9.97 ± 0.53 and in GS from 1.15 ± 0.03 to 2.39 ± 0.04. The OCT and ARG enzymes remained constant in H. unitaeniatus (N = 6), but GS increased from 1.49 ± 0.02 to 2.06 ± 0.03. Although these species are very closely related and share the same environment, their biochemical strategies in response to exposure to air or to increased plasma ammonia are different.

【 授权许可】

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