In vitro cytotoxicity of the LDE: daunorubicin complex in acute myelogenous leukemia blast cells

Brazilian Journal of Medical and Biological Research

P.e. Dorlhiac-llacer²  M.v. Marquezini¹  O. Toffoletto¹  R.c.g. Carneiro¹  R.c. Maranhão¹  D.a.f. Chamone² 

[1] ,Universidade de São Paulo Faculdade de Medicina Departamento de Hematologia e HemoterapiaSão Paulo SP ,Brasil

关键词: cytotoxicity;    acute myelogenous leukemia;    daunorubicin;    low-density lipoprotein;    LDE complex;    RT-PCR;   

DOI  :  10.1590/S0100-879X2001001000004

来源: SciELO

【 摘 要 】

Acute myelogenous leukemia (AML) blast cells show high-affinity degradation of low-density lipoprotein (LDL), suggesting an increased expression of cellular LDL receptors. LDE is a lipid microemulsion easily synthesized in vitro which is known to mimic the metabolic pathway of LDL. We used LDE as a carrier for daunorubicin and assayed the cytotoxicity of the complex using AML blast cells since RT-PCR analysis showed that AML cells express LDL receptor mRNA. The LDE:daunorubicin complex killed 46.7% of blast cells and 20.2% of normal bone marrow cells (P<0.001; Student t-test). Moreover, this complex destroyed AML blast cells as efficiently as free daunorubicin. Thus, LDE might be a suitable carrier of chemotherapeutic agents targeting these drugs to neoplastic cells and protecting normal tissues.

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