Effects of a neuronal nitric oxide synthase inhibitor on lipopolysaccharide-induced fever

Brazilian Journal of Medical and Biological Research

C.a.a. Perotti²  M.s. Nogueira²  J. Antunes-rodrigues¹  E.c. Cárnio² 

[1],Universidade de São Paulo

关键词: nitric oxide;    7-nitroindazole;    body temperature;    LPS;    L-NAME;   

DOI  :  10.1590/S0100-879X1999001100008

来源: SciELO

【 摘 要 】

It has been demonstrated that nitric oxide (NO) has a thermoregulatory action, but very little is known about the mechanisms involved. In the present study we determined the effect of neuronal nitric oxide synthase (nNOS) inhibition on thermoregulation. We used 7-nitroindazole (7-NI, 1, 10 and 30 mg/kg body weight), a selective nNOS inhibitor, injected intraperitoneally into normothermic Wistar rats (200-250 g) and rats with fever induced by lipopolysaccharide (LPS) (100 µg/kg body weight) administration. It has been demonstrated that the effects of 30 mg/kg of 7-NI given intraperitoneally may inhibit 60% of nNOS activity in rats. In all experiments the colonic temperature of awake unrestrained rats was measured over a period of 5 h at 15-min intervals after intraperitoneal injection of 7-NI. We observed that the injection of 30 mg/kg of 7-NI induced a 1.5oC drop in body temperature, which was statistically significant 1 h after injection (P<0.02). The coinjection of LPS and 7-NI was followed by a significant (P<0.02) hypothermia about 0.5oC below baseline. These findings show that an nNOS isoform is required for thermoregulation and participates in the production of fever in rats.

【 授权许可】

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