Modulation of staphylokinase-dependent plasminogen activation by mono- and divalent ions

Brazilian Journal of Medical and Biological Research

A. Yarzábal¹  R.l. Serrano²  J. Puig¹ 

关键词: staphylokinase;    plasminogen activation;    fibrinolysis;   

DOI  :  10.1590/S0100-879X1999000100005

来源: SciELO

【 摘 要 】

The effect of several ions (Cl-, Na+, K+, Ca2+) on the rate of plasminogen (Pg) activation by recombinant staphylokinase (rSTA) is reported. Both monovalent and divalent ions affect the rate at which Pg is activated by rSTA, in a concentration-dependent manner (range 0-100 mM). In almost all cases, a decrease of the initial velocity of activation was observed. Cl- showed the most striking inhibitory effect at low concentrations (64% at 10 mM). However, in the presence of a fibrin surface, this inhibition was attenuated to 38%. Surprisingly, 10 mM Ca2+ enhanced the Pg activation rate 21% when a polymerized fibrin matrix was present. These data support the idea that ions can modulate the rate of Pg activation through a mechanism that may be associated with changes in the molecular conformation of the zymogen. This effect is strongly dependent on the presence of a fibrin clot.

【 授权许可】

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