Immunohistochemical study of genital and extragenital forms of canine transmissible venereal tumor in Brazil

Pesquisa Veterinária Brasileira

Mariana B. Mascarenhas²  Paulo V. Peixoto¹  Regina R. Ramadinha¹  Elise M. Yamasaki²  Samay Z.r. Costa²  David Driemeier¹  Luciana Sonne¹  Ticiana N. França¹ 

[1] ,UFRRJ Programa de Pós-Graduação em Medicina Veterinária Seropédica RJ

关键词: Canine transmissible venereal tumor;    immunohistochemistry;    round cell tumor;    dog;    pathology;    Tumor venéreo transmissível;    imuno-histoquímica;    tumor de células redondas;    cão;    patologia;   

DOI  :  10.1590/S0100-736X2014000300009

来源: SciELO

【 摘 要 】

Aiming to provide insight and discussing the problems related to the diagnosis and differential diagnosis of canine transmissible venereal tumor (CTVT), especially in its extragenital form, immunohistochemical evaluation was performed and a comparison was established by analysis of the microscopic appearance of 10 genital CTVTs and 13 exclusively extragenital CTVTs previously diagnosed by cytology and histopathology. CTVTs samples were incubated with biotinylated antibodies raised against specific membrane (anti-macrophage) and cytoplasmic antigens (anti-lysozyme, anti-S-100 protein, anti-vimentin and anti-CD18) and subsequently developed using streptavidin-biotin peroxidase and streptavidin-biotin-alkaline phosphatase methods. A strong reactivity with the anti-vimentin antibody was found in 100% of the tumors tested (22/22). No reactivity was found for the anti-lysozyme, anti-macrophage, anti-S-100 protein and anti-CD18. No histopathological or immunoreactivity differences between genital and extragenital CTVTs were found. These findings do not corroborate the hypothesis of histiocytic origin of CTVT (no reactivity to anti-lysozyme, anti-macrophage and anti-CD 18 antibodies). In addition, the antibody panel used is useful to narrow the differential diagnosis for lymphomas, histiocytic tumors, amelanotic melanomas, and poorly differentiated epithelial neoplasias, among others.

【 授权许可】

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