Detection of specific antibodies in cows after injection of PPD

Pesquisa Veterinária Brasileira

Carla D. Marassi²  Carlos Zarden²  Walter Oelemann¹  Walter Lilenbaum² 

[1] ,Universidade Federal Fluminense Departamento de Bacteriologia e Parasitologia Laboratório de Bacteriologia VeterináriaNiterói RJ ,Brazil

关键词: ELISA;    tuberculosis;    cattle;    MPB70;    MPB83;    interference;    ELISA;    tuberculose;    bovinmos;    MPB70;    MPB83;    Interferência;   

DOI  :  10.1590/S0100-736X2014000300007

来源: SciELO

【 摘 要 】

The diagnosis of bovine tuberculosis aims to identify the immune response against mycobacterial antigens. Although Single Intradermal Comparative Cervical Tuberculin test (SICCT) is broadly used for first identification of the disease, the performance of ELISAs has been investigated for diagnosis improvement. The present study expected to find out the influence of intradermal skin tests on the results of ELISAs using the recombinant proteins MPB70 and MPB83 as antigens on cows from a naturally infected herd. Results were analyzed by the F-test, Mann-Whitney and Friedman tests Although comparable to both proteins, results showed that positive animals presented a tendency of augment reactivity to MPB70, representing a tendency for a booster effect, but not to MPB83.

【 授权许可】

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