Induction of immune response in broiler chickens immunized with recombinant FliC and challenged by Salmonella Typhimurium

Pesquisa Veterinária Brasileira

Ana Angelita S. Baptista²  Tais C. Donato²  Edmárcia E. Souza¹  Guilherme A.m. Gonçalves²  Keila C.o.d. Garcia²  João C.z. Rodrigues²  Júlio L. Sequeira²  Raphael L. Andreatti Filho² 

[1] ,Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Departamento de Clínica VeterináriaBotucatu SP ,Brazil

关键词: Salmonella Typhimurium;    FliC;    flagellin;    immune response;    broiler chickens;    Salmonella Typhimurium;    FliC;    flagelina;    resposta imune;    frangos de corte;   

DOI  :  10.1590/S0100-736X2013001000007

来源: SciELO

【 摘 要 】

The study examined (1) the immune response in broiler chickens after oral immunization with recombinant flagellin (rFliC) from Salmonella Typhimurium conjugated with sodium alginate microparticles, and the immune response enhancement in association with recombinant cholera toxin B subunit protein (rCTB) and pool of Lactobacillus spp. (PL). The immune responses were evaluated by dosage of IgY serum and IgA from intestinal fluid and immunostaining of CD8+ T lymphocytes in the cecum. The immunized animals were challenged with Salmonella Typhimurium (ST) 21 days after treatment. In all immunized groups, a significant increase (p<0.05) was observed in IgA levels (μg/mL), especially three weeks after immunization. The serum IgY levels (μg/mL) were little affected by the treatments and differed significantly among groups only in the second post-immunization week (p<0.05). After the challenge, the number of CD8+ T cells differed significantly between the treatments and negative control. Retrieval of Salmonella Typhimurium was not detected at 48 hours after the challenge in T2 (rFliC+rCTb), T3 (rFliC+PL) and T4 (rFliC+rCTB PL). The rFliC administered orally with or without rCTB and Lactobacillus spp. produces significant induction of humoral immune response, and the immunized chickens were more effective in eliminating Salmonella after challenge.

【 授权许可】

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