Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)

Pesquisa Veterinária Brasileira

Patrícia C. Sousa¹  Erika A.a. Santos¹  Ana L.p. Souza¹  Gabriela L. Lima¹  Felipe F.p.c. Barros¹  Moacir F. Oliveira¹  Alexandre R. Silva¹ 

[1] ,Universidade Federal Rural do Semi-Árido Laboratório de Conservação de Germoplasma Animal Mossoró RN ,Brazil

关键词: Pecari tajacu;    staining;    sperm morphology;    sperm morphometry;    Pecari tajacu;    coloração;    morfologia espermática;    morfometria espermática;   

DOI  :  10.1590/S0100-736X2013000700014

来源: SciELO

【 摘 要 】

The aim of this study was to compare different staining methods for the evaluation of sperm morphology by light microscopy and also to describe the morphometry of the entire sperm in collared peccaries (Pecari tajacu). Semen from 10 males was obtained by electroejaculation and evaluated for sperm motility, vigor, and concentration. Semen smears were prepared through three different staining methods: Bengal rose, brome-phenol blue, and eosin-nigrosin. Smears were evaluated under light microscopy and sperm morphologic alterations were determined in percentage. In addition, sperm morphometric analysis was conducted by light microscopy coupled to image analyzer software. The smears stained with Bengal Rose provide the best results for the visualization of the sperm tail, midpiece, and head. The use of eosin-nigrosin stain did not allow an adequate impregnation, and some sperm presented a few contrasts with the background. A higher incidence of bent coiled tails was verified in the use of brome-phenol blue staining (P<0.05). Through morphometric evaluation, it was observed that the tail occupies the greatest proportion (89%) of the sperm which presents a discretely elongated head. According to the results, the use of the Bengal Rose stain is recommended for the morphologic evaluation of the collared peccary sperm.

【 授权许可】

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