Molecular characterization of group A bovine rotavirus in southeastern and central-western Brazil, 2009-2010

Pesquisa Veterinária Brasileira

Fernanda D.f. Silva²  Fábio Gregori¹  Ana C.s. Gonçalves²  Samir Issa Samara²  Maria G. Buzinaro² 

[1] ,Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias e Veterinárias Departamento de Medicina Veterinária Preventiva e Reprodução AnimalJaboticabal SP ,Brazil

关键词: Rotavirus;    diarrhea;    genotypes;    cattle;    Brazil;    Rotavírus;    diarreia;    genotipos;    bovinos;    Brasil;   

DOI  :  10.1590/S0100-736X2012000300010

来源: SciELO

【 摘 要 】

Rotavirus is an important cause of neonatal diarrhea in humans and several animal species, including calves. A study was conducted to examine 792 fecal samples collected from calves among 65 dairy and beef herds distributed in two of Brazil's major livestock producing regions, aiming to detect the occurrence of rotavirus and perform a molecular characterization of the rotavirus according to G and P genotypes in these regions. A total of 40 (5.05%) samples tested positive for rotavirus by the polyacrylamide gel electrophoresis (PAGE) technique. The molecular characterization was performed by multiplex semi-nested RT-PCR reactions, which indicated that the associations of genotypes circulating in herds in Brazil's southeastern region were G6P[11], G10P[11], G[-]P[5] + [11], G[-]P[6] in the state of São Paulo and G6P[11], G8P[5], G11P[11], G10P[11] in the state of Minas Gerais. In the central-western region, the genotypes G6P[5] + [11], G6P[5], G8P[-], G6P[11], G [-] P[1], G[-] P[11], and G[-] P[5] were detected in the state of Goiás, while the genotypes G6P[5], G8[P11], G6[P11], G8[P1], G8[P5], G6[P1] were circulating in herds in the state of Mato Grosso do Sul. The genotypic diversity of bovine rotavirus found in each region under study underlines the importance of characterizing the circulating samples in order to devise the most effective prophylactic measures.

【 授权许可】

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